【摘要】 为得到性状优良且具有生防潜力的虫生真菌,本试验对已获得的虫生真菌进行了基础生物学测定并对6种主要病原菌抑菌活性进行测定。结果表明:菌株FNS5和FYS3的最适生长温度分别为25～28℃和28～30℃,菌株FYS3的最适产孢温度较广,为20～30℃。菌株FNS5的最适产孢温度为20～25℃。48h镜检菌株FNS5在25℃处理下萌发率最高为68%,与其他处理差异显著。菌株FNS5、FYS3均表现出广谱且较强的抑制作用,平皿对峙试验,金龟子绿僵菌FYS3对水稻稻瘟病的抑制率达47.13%,琼脂扩散法试验,金龟子绿僵菌FNS5、FYS3的代谢产物对番茄灰霉病菌具有拮抗作用,抑菌圈直径分别为(3.22±0.19)cm和(3.51±0.29)cm,是两株具有进一步研究价值的生防菌株。更多还原

【Abstract】 In order to obtain entomogenous fungi with excellent traits and potential for biocontrol,this experiment carried out basic biological assays on the obtained entomogenous fungi and the antibacterial activity of6 main species pathogens.The results showed that the optimal growth temperature of strain FNS5 and FYS3 were 25-28℃and 28-30℃,respectively.The optimal sporulation temperature of strain FYS3 was wider,which was 20-30℃ and strain FNS5 was 20-25℃．The germination rate of strain FNS5 under the 48 hmicroscopic examination was 68% at 25 ℃,which was significantly different from other treatments.Strains FNS5 and FYS3 both showed broad-spectrum and strong inhibitory effects.In the plate confrontation test,the inhibition rate of Metarhizium anisopliae FYS3 on Pyricularia grisea reached 47.13%.The agar diffusion test showed the metabolism of Metarhizium anisopliae FNS5 and FYS3 had an antagonistic effect on Botrytis cinerea,and the diameters of the inhibition zone were(3.22±0.19)cm and(3.51±0.29)cm,respectively.So they have further biocontrol research value.更多还原