【摘要】 目的:探究细胞周期蛋白依赖性激酶抑制因子3(CDKN3)和叉头框蛋白A1(FOXA1mRNA在直肠癌中的表达水平及其临床意义。方法:选取2017年11月至2020年3月在唐山市协和医院和解放军陆军第82集团军医院就诊的94例直肠癌患者作为观察组,另选取同时期在医院进行常规体检的健康人群95例,作为对照组;采用实时荧光定量PCR(qRT-PCR)检测直肠癌患者血清中的CDKN3和FOXA1 mRNA表达情况;分析高、低级别直肠癌患者血清CDKN3和FOXA1 mRNA的表达水平;分析CDKN3、FOXA1 mRNA水平与直肠癌患者临床病理特征的关系;预测CDKN3和FOXA1 mRNA水平对高、低直肠癌患者的诊断价值。结果:观察组血清中CDKN3与FOXA1 mRNA的表达水平明显高于对照组(P<0.05);高级别直肠癌患者血清中CDKN3、FOXA1 mRNA的表达量明显高于低级别直肠癌患者(P<0.05);直肠癌患者血清中CDKN3、FOXA1 mRNA表达水平与TNM分期、分化程度和淋巴结是否转移有关(P<0.05); CDKN3 mRNA诊断高、低级别直肠癌的ROC曲线下面积为0.839,截断值为5.317,灵敏度为63.8%,特异性为94.4%; FOXA1 mRNA诊断高、低级别直肠癌的ROC曲线下面积为0.853,截断值为0.740,灵敏度为70.7%,特异性为97.2%。结论:直肠癌患者血清中CDKN3、FOXA1 mRNA表达量上升,且高级别直肠癌患者二者表达量显著高于低级别直肠癌患者,CDKN3、FOXA1 mRNA参与直肠癌病情进程与发展,对高、低级别直肠癌诊断具有一定价值。更多还原

【Abstract】 Objective: To investigate the expressions and clinical significance of cyclin dependent kinase inhibitor 3( CDKN3) and forked head box A1( FOXA1) mRNA in rectal cancer. Methods: 94 rectal cancer patients in Tangshan Union Hospital and the 82 nd Army Hospital of the PLA from November 2017 to March2020 were used as the observation group,and 95 healthy people who had routine physical examination in our hospital in the same period as the control group; the expressions of CDKN3 and FOXA1 mRNA in serum of patients with rectal cancer were detected by real-time fluorescent quantitative PCR( QRT PCR); the expression levels of CDKN3 and FOXA1 mRNA in serum of patients with high and low grade rectal cancer were analyzed;the relationships between the levels of CDKN3 and FOXA1 mRNA and the clinicopathological characteristics of rectal cancer patients were analyzed; and the diagnostic values of CDKN3 and FOXA1 mRNA levels in patients with high and low rectal cancer were predicted. Results: The expression levels of CDKN3 and FOXA1 mRNA in the observation group were significantly higher than those in the control group( P <0.05); the expressions of CDKN3 and FOXA1 mRNA in the serum of patients with high rectal cancer were significantly higher than those of patients with low rectal cancer( P <0.05); in the serum of patients with rectal cancer the expression levels of CDKN3 and FOXA1 mRNA were related to TNM stage,differentiation degree and lymph node metastasis( P < 0. 05); the area under ROC curve of CDKN3 mRNA in the diagnosis of high and low grade rectal cancer was 0.839,the cutoff value was 5.317,the sensitivity was 63.8%,and the specificity was94.4%; the area under ROC curve of FOXA1 mRNA in the diagnosis of high and low grade rectal cancer was0.853,the cutoff value was 0.740,the sensitivity was 70.7%,and the specificity was 97.2%. Conclusions:The expressions of CDKN3 and FOXA1 mRNA in serum of patients with rectal cancer are increased,in addition,the expressions of them in patients with high-grade rectal cancer are significantly higher than those in patients with low-grade rectal cancer,CDKN3 and FOXA1 mRNA are involved in the process and development of rectal cancer,and have certain values in the diagnosis of high and low grade rectal cancer.更多还原