【摘要】 为探讨镉（Cd）胁迫下凡纳滨对虾（Litopenaeus vannamei）血细胞的分子响应,以不同浓度的Cd²⁺（0,0.5 mg/L和5 mg/L）进行胁迫,于不同胁迫时间取血淋巴,测定血细胞中Trx 2、Grx 2、Grx 3和MGST 3的基因表达量变化。结果显示,Trx 2、Grx 3和MGST 3表达量在胁迫的中后期显著上调,Grx 2表达量在5 mg/L Cd²⁺胁迫6 h开始即有显著的升高,并持续至48 h。结果表明在Cd胁迫作用下,对虾血细胞中上调这四个氧化还原与抗氧化相关基因的表达水平,以进行胁迫防御;Grx 2对Cd胁迫较为敏感,在整个胁迫过程发挥作用,Trx 2、Grx 3和MGST 3主要在胁迫的中后期发挥作用;基因的表达响应具有一定浓度效应。更多还原

【Abstract】 To investigate the molecular response of haemocytes of the shrimp Litopenaeus vannamei to Cd stress, gene expression levels of Trx 2,Grx 2,Grx 3 and MGST 3 were measuredin haemocytes from shrimp exposed to Cd²⁺（0,0.5 mg/L and 5 mg/L） for different times.Results showed that the expression levels of Trx 2,Grx 3 and MGST 3 were significantly up-regulated in the middle and late stages of the stress, and the expression level of Grx 2 was significantly increased after 6 h exposure to 5 mg/L Cd²⁺,and this level continued to 48 h.These results indicate that expression of these four redox and antioxidant related genes in haemocytes of L.vannamei are up-regulated to protect against Cd stress.Grx 2 is sensitive to Cd stress and plays a role in the whole stress process.Trx 2,Grx 3 and MGST 3 mainly play a role in the middle and late stages of the stress.The gene expression response has a certain concentration effect.更多还原