【摘要】 建立地黄(Rehmannia glutinosa)不同部位总环烯醚萜苷的含量测定方法并检测7份地黄种质的总环烯醚萜苷含量。以梓醇为对照品,采用硫酸-香草醛显色法于510 nm对地黄叶片和根中的总环烯醚萜苷含量进行测定。结果表明,该方法在0.01～0.25 mg/mL线性关系良好,回归方程A=1.191 7ρ-0.003 5,相关系数R2=0.999 9。样品平均回收率为100.65%,RSD为1.61%。地黄叶片总环烯醚萜苷的含量普遍高于根含量。试验建立的方法简单快捷、准确,可以用于对地黄根和叶片中总环烯醚萜苷进行含量分析和质量评价。更多还原

【Abstract】 To establish a method for determination of total iridoid glutinoside in the extract of Rehmannia glutinosa and measure thecontent of total iridoid glutinoside in seven Rehmannia glutinosa germplasms. The Sulfo-Phospho-Vanillin method was applied to com-pare the content of total iridoid glutinoside in Rehmannia glutinosa. The absorbance was detected at 510 nm. The results showed that astand curve using catalpol was constructed at a concentration rang of 0.01～0.25 mg/m L. The regression equation was A=1.191 7ρ-0.003 5,the correlation coefficient is 0.999 9. It showed that the content of total iridoid glutinosides in leaves was usually higher than that inroots. The method established in this study is table,simple and rapid for determination of the total iridoid content in Rehmannia glutinosa,and it will provide convenience for content evaluation studies on samples of Rehmannia glutinosa.更多还原