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干旱锻炼对小麦幼苗期抗氧化特性的影响

Priming of Drought on the Antioxidant Characteristics of Wheat Seedlings

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【作者】 万雪洁张艳师长海周宣材孙新灵李松坚张守福刘义国

【Author】 WAN Xuejie;ZHANG Yan;SHI Changhai;ZHOU Xuancai;SUN Xinling;LI Songjian;ZHANG Shoufu;LIU Yiguo;Qingdao Agricultural University,Shandong Province Key Laboratory of Dry Farming Technology, Dry Land Crop and Water Innovation Team;Agriculture and Rural Bureau of Yanzhou District;Qingdao Agricultural Technology Extension Center;Qingdao Hailiyuan Biotechnology Limited Company;

【通讯作者】 刘义国;

【机构】 青岛农业大学山东省旱作农业技术重点实验室旱作节水创新团队济宁市兖州区农业农村局青岛市农业技术推广中心青岛海力源生物科技有限公司

【摘要】 为探究干旱锻炼对小麦幼苗期抗氧化特性的影响,设置对照处理和干旱锻炼处理,在一心一叶时用PEG6000营养液进行干旱锻炼,之后恢复霍格兰氏全营养液,在三叶一心时分别进行干旱胁迫处理,观测幼叶中与抗氧化特性相关物质的动态变化。研究发现,随干旱程度及胁迫时间的增加,叶片SOD、POD活性、可溶性蛋白不同程度的减小,MDA、可溶性糖含量显著升高;与对照相比,干旱锻炼后叶片SOD、POD活性、可溶性蛋白及降低程度较小,可溶性糖含量升高程度较大,MDA升高程度较小;不同干旱程度下,25%PEG6000胁迫下干旱锻炼小麦的抗氧化能力较35%PEG6000胁迫下较高。由此说明,小麦经过一定浓度的干旱锻炼能够明显增强其对干旱环境的适应能力,有利于幼苗期的生长,也为小麦的抗逆性研究提供理论支持。

【Abstract】 In order to explore the effects of drought priming on antioxidant properties of wheat seedlings, control treatment and drought training treatment were set up in this experiment. PEG6000 nutrient solution was used for drought training when one heart and one leaf, and then Hoagland’s total nutrient solution was restored. When three leaves are one heart, the drought stress is treated separately, and the dynamic changes of the substances related to the antioxidant properties in the young leaves were observed. The results showed that with the increase of drought degree and stress time, SOD,POD activity and soluble protein decreased, but MDA and soluble sugar content increased significantly. Compared with the control, the leaf SOD, POD activity and soluble protein decreased less after drought exercise, the soluble sugar content increased more, and the MDA increased less. Under the different drought stress, the antioxidative capacity of drought-stressed wheat under 25% PEG6000 stress was higher than that of 35% PEG6000. This showed that a certain concentration of drought training in wheat can significantly enhance its ability to adapt to drought conditions, which was beneficial to the growth of the seedling stage and provides theoretical support for the research on wheat resistance.

【基金】 山东省农业重大应用技术创新项目(SD2019ZZ003);山东省重点研发计划(2019GNC106012);山东省高等学校青创科技支持计划(2019KJF013);青岛农业大学高层次人才科研基金(6631114308);青岛市科技惠民引导专项(21-1-4-ny-31-nsh)
  • 【文献出处】 华北农学报 ,Acta Agriculturae Boreali-Sinica , 编辑部邮箱 ,2021年S1期
  • 【分类号】S512.1
  • 【被引频次】2
  • 【下载频次】279
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