【摘要】 为探究不同核背景下线粒体复合体活性对玉米C型雄性不育系育性的影响,明确其与花药败育的关系。以同质异核不育系K932S、K169S、近等基因恢复系K932R和保持系K169为材料,采用植物线粒体呼吸链复合体酶联免疫分析试剂盒对花药发育不同时期的5个线粒体复合体活性进行测定比较。结果表明,除花粉母细胞时期复合体Ⅱ、Ⅳ外,其余时期不育细胞质复合体Ⅰ、Ⅱ、Ⅳ活性在K169核背景下显著高于K932,绝大多数时期复合体Ⅲ、Ⅴ的活性则相反,但存在恢复基因时,K932R可改变部分时期不育细胞质不同线粒体复合体的活性。在二分体时期,不育细胞质复合体Ⅱ、Ⅲ、Ⅴ活性显著低于正常可育胞质,而其他多数时期5个复合体的活性则相反。由此可见,在花药发育不同时期,核背景对玉米CMS-C不同线粒体复合体活性均有显著影响;相同核背景下,二分体时期不育细胞质复合体Ⅱ、Ⅲ、Ⅴ活性比正常细胞质显著降低,可能与CMS-C花药败育密切相关。更多还原

【Abstract】 In order to explore the influence of mitochondrial complex activity on the fertility of C-type male sterile lines in maize under different nuclear backgrounds, and to clarify its relationship with anther abortion. Plant mitochondrial respiratory chain complex enzyme linked immunoassay kit was used to determine the activities of 5 mitochondrial complex in different stages of anther development, using homogeneous heterogenic male sterile lines K932 S and K169 S,restorer line K932 R and maintainer line K169 as materials. Results showed that in addition to the period of pollen mother cell complex Ⅱ,Ⅳ,the remaining period of sterile cytoplasm complex Ⅰ,Ⅱ,Ⅳ activity under the background of K169 nuclear was significantly higher than K932,most of the time the activity of complex Ⅲ,Ⅴ,on the other hand, when there was a restoring genes but K932 R might change the part of the activity of sterile cytoplasm during different mitochondrial complex. The activity of sterile cytoplasmic complexes Ⅱ,Ⅲ and Ⅴ was significantly lower than that of normal fertile cytoplasm at the diad stage, while the activity of five complexes at most other stages was the opposite. It can be seen that the nuclear background have a significant effect on the activity of different mitochondrial complexes of CMS-C in maize at different anther development stages. Under the same nuclear background, the activity of cytoplasmic complexes Ⅱ,Ⅲ and V at the diploid stage was significantly lower than that of normal cytoplasm, which might be closely related to CMS-C drug abortion.更多还原