【摘要】 为了探究大麻二酚酸合成酶基因(CBDAS)家族在大麻中的功能,利用生物信息学方法对大麻二酚酸合成酶基因(CBDAS)进行了全基因组鉴定,并系统分析其理化特性、进化发育、基因结构、启动子顺式结合元件及表达模式。结果表明,大麻CBDAS基因家族包含5个成员,仅分布在2,7号染色体上;理化性质分析显示,大麻CBDAS基因家族编码的氨基酸数目为541～545 aa,分子质量为介于61.49～62.41 ku,等电点为6.90～9.00;系统进化分析显示,来自植物、动物和微生物的33个CBDAS基因可分为3个家族,多数CsCBDAS基因家族成员属于同一亚家族;启动子区顺式作用元件预测表明,CsCBDAS基因家族成员均富含光响应元件;组织特异表达分析显示,CsCBDAS1和CsCBDAS2在雌蕊中表达最高,CsCBDAS4、CsCBDAS5在根中表达量最高;大麻CBDAS基因在高大麻二酚(CBD)和低CBD含量品种的表达模式不同,CsCBDAS1表达量在高大麻二酚(CBD)品种中高于低CBD含量品种;外源重金属Cd处理CsCBDAS4和CsCBDAS5基因表达量显著上调表达;CsCBDAS1、CsCBDAS2及CsCBDAS5表达量在黑暗和光照处理间表现出显著差异,光照处理下,CBDAS1基因表达量显著低于黑暗处理,而CsCBDAS2及CsCBDAS5的表达量高于黑暗处理。这些基因可能参与大麻的生长发育与大麻素的合成,该研究为后续大麻CBDAS基因家族的功能研究奠定了基础。更多还原

【Abstract】 In order to reveal the functions of cannabidiolic acid synthase genes(CBDAS),the CBDAS genes in cannabis were identified using bioinformatics methods, and their physical and chemical characteristics, evolutionary relationship, gene structure, the cis-acting elements and transcript patterns were analyzed.Totally, 5 CBDAS genes were identified, and they distributed only on 2 chromosome and 7 chromosome.Their amino acid sequence size, molecular weight and isoelectric point were 541-545 aa, 61.49-62.41 ku, and 6.90-9.00,respectively.Evolution analysis showed that 33 CBDAS gene family members from plants, animals and microorganism were divided into three subclasses, and most of CsCBDAS belonged to the same subclass.The promoters of all CsCBDAS genes were rich in cis-acting elements related to light response.In addition, tissue-specific expression analysis showed that CsCBDAS1 and CsCBDAS2 preferred expressed in female flower and CsCBDAS4 and CsCBDAS5 preferred expressed in root.Moreover, the CsCBDAS members showed different expression patterns between the high-CBD cultivar and low-CBD cultivar, and the expression level of CsCBDAS1 in a high-CBD cultivar was higher than that in a low-CBD cultivar.Exogenous cadmium treatment could increase the exprssion levels of CsCBDAS4 and CsCBDAS5 with cadmium treatment were significantly higher than those without cadmium treatment.The transcript levels of CsCBDAS1,CsCBDAS2 and CsCBDAS5 exhibited significant difference between in light treatment and in darkness treatment, the transcript levels of CsCBDAS1 in light treatment was significantly lower than that in darkness treatment, but the expression levels of CsCBDAS2 and CsCBDAS5 were higher than those in darkness treatment.The CBDAS may involved in the growth and development of cannabis and cannabinoid synthesis, and this study laid the foundation for the further elucidation of the functions of CBDAS genes in cannabis.更多还原