【摘要】 药效物质与药物靶标的相互作用是其生物活性的基础,利用药物靶标亲和选择性"钩钓"其特异性配体,可以实现复杂提取物中天然活性物质分析的"精准制导".亲和超滤是一种以上述原理为基础,将亲和捕获与超滤分离相结合以实现化合物高通量筛选的技术,具有快速、简单、高效等特点,常与液质联用等分析技术联合使用,克服了天然活性物质筛选研究面临的诸多问题,近年来被广泛应用于天然活性物质的快速靶向筛选.本文从亲和超滤技术本身的原理、影响因素及与其他分析技术的联用情况等方面,详细综述了亲和超滤技术的特点和应用现状,以期为构建天然活性物质的快速筛选策略提供科学思路和依据.更多还原

【Abstract】 The interaction between medicinal substance and drug target is the basis of biological activity for the medicinal substance,and“precise guidance”of natural bioactive compounds from complex extracts can be achieved by the selective affinity of drug target,just like “fishing”.Affinity ultrafiltration is a technology that combines affinity capture and ultrafiltration separation for high-throughput screening of bioactive compounds based on the above principles,which is often coupled with liquid chromatographymass spectrometry,overcoming lots of problems in discovering bioactive natural products.This paper reviewed in detail the characteristics and application status of affinity ultrafiltration technology,influencing factors,and the combination with other analytical technologies,in order to provide scientific ideas and basis for the construction of rapid screening strategies for natural active substances.更多还原