【摘要】 目的探究隐丹参酮对大鼠吗啡条件性位置偏爱（CPP）效应形成的影响及其抗吗啡CPP的可能作用部位和机制。方法建立吗啡CPP效应模型观察隐丹参酮对其影响;实时荧光定量PCR和Western Blot法检测大鼠成瘾相关脑区信号分子水平变化。结果:中剂量隐丹参酮40 mg·kg^-1干预组可拮抗吗啡CPP效应形成;该组内侧前额叶皮层及海马区BDNF的表达水平与吗啡组比较明显降低。结论中剂量隐丹参酮40 mg·kg^-1可抑制吗啡CPP效应形成;这种抑制可能与其减少BDNF信号分子表达水平有关。更多还原

【Abstract】 OBJECTIVE To investigate the effect of cryptotanshinone on the formation of morphine-induced conditioned place preference（CPP） in rats and the possible role and mechanism.MEHTODS Intraperitoneal injection of cryptotanshinone was used to observe its effect on the formation of CPP induced by morphine;Western Blot and Real-time quantitative PCR were used to detect the level of signal molecules in related brain regions.RESULTS Cryptotanshinone at a dose of 40 mg·kg^-1 could inhibit the formation of morphine CPP;Compared with morphine group,the level of BDNF in the mPFC and Hippo were significantly decreased in the cryptotanshinone at a dose of 40 mg·kg^-1 intervene group by western-blot and RT-PCR.CONCLUSION Cryptotanshinone at a dose of 40 mg·kg^-1 showed inhibiting the formation of morphine-induced CPP in rats;The inhibition may be related to decrease the level of BDNF signaling molecules.更多还原