【摘要】 目的通过建立大鼠心肌缺血再灌注损伤（I/RI）的模型,探讨槲寄生联合远程缺血后适应(RIPOC对大鼠心肌是否具有协同保护作用。方法 50只SD大鼠随机分5组,每组10只:假手术组（Sham组）、缺血再灌注损伤组（I/RI组）、槲寄生组、远程缺血后适应组（RIPOC组）、联合组（槲寄生+RIPOC组）。记录开胸前基础值（T0）,缺血10 min（T₁）,30 min（T₂）,和再灌后30 min（T₃）,60 min（T₄）,120 min（T₅）的心率（HR）、平均动脉压（MAP）。再灌结束后检测各组大鼠血清肌酸激酶（CK）、肌酸激酶心肌型同工酶（CK-MB）、乳酸脱氧酶（LDH）的含量的变化,并测定心肌缺血、梗死程度。结果 HR:T₁至T₅时刻,与I/RI组比较,各干预组（槲寄生组、RIPOC组和联合组,下同）HR相对较高（P <0.05,P <0.01）。MAP:T₂至T₅时刻,与I/RI组比较,各干预组大鼠MAP相对较高（P <0.01）。心肌酶指标:与I/RI组比较,各干预组大鼠的三种血清酶浓度均有所降低（P <0.01）。心肌梗死程度:与I/RI组比较,各干预组大鼠的梗死程度均明显降低（P <0.01）。以上结果中,联合组大鼠的效果最好。结论槲寄生和RIPOC联合应用可以改善I/RI大鼠血流动力学,还可以减少心肌酶的释放,改善再灌注后心肌损伤,对I/RI的心肌有一定的保护作用。更多还原

【Abstract】 Objective To investigate whether mistletoe combined with remote ischemic post-conditioning（RIPOC） has synergistic protective effects on rat myocardium by establishing an ischemia-reperfusion injury（I/RI） model. Methods A total of 50 SD rats were randomly divided into the sham operation group（the sham group, n = 10）, the ischemia-reperfusion injury group（the I/RI group, n=10）, the mistletoe group（n = 10）, the remote ischemic postconditioning group（the RIPOC group, n=10） and the combined group（the mistletoe combined with RIPOC group, n=10）. Heart rate（HR） and mean arterial pressure（MAP） were recorded before thoracotomy（T₀）, 10 min（T₁） and 30 min（T₂） after ischemia, and 30 min（T₃）, 60 min（T₄） and 120 min（T₅） after reperfusion. At the end of reperfusion, the changes of serum creatine kinase（CK）, creatine kinase isoenzyme MB（CK-MB） and Lactic dehydrogenase（LDH） were detected, and the degree of myocardial ischemia and infarction was measured. Results For HR, from T₁ to T₅, compared with the I/RI group, the HR of each intervention group（the mistletoe group, the RIPOC group and the combined group, similarly hereinafter） was relatively higher（P < 0.05,P < 0.01）. For MAP, from T₂ to T5, compared with the I/RI group, the MAP of rats in each intervention group was relatively higher（P < 0.01）. For myocardial enzymes indices, compared with the I/RI group, the concentration of three serum enzymes in each intervention group of rats all decreased to some extent（P < 0.01）. For the degree of myocardial infarction, compared with the I/RI group, the degree of myocardial infarction in each intervention group of rats all decreased significantly（P < 0.01）. Among the above results, the combined group of rats has the best effect. Conclusion The combined application of mistletoe and RIPOC can improve the hemodynamics of rats in the I/RI group, reduce the release of myocardial enzymes, improve myocardial injury after reperfusion, and protect the myocardium of rats in the I/RI group to some extent.更多还原