【摘要】 目的:探讨粪菌移植疗法(FMT)对大鼠脊髓损伤(SCI)后的作用。方法:将36只SD大鼠随机分为对照组(A组)、SCI组(B组)、FMT组(C组),每组12只。A组仅手术暴露,不进行击打,B组和C组用改良Allen’s击打法建立SCI模型。C组灌胃给予粪菌液,连续14 d。对16S rDNA V3-V4高度保守区进行HiSeq平台测序,比较SCI后肠道菌群变化,Basso-BeattieBresnahan(BBB)评分评估大鼠后肢情况,酶联免疫吸附试验(ELISA)法检测脊髓组织IL-17、IL-4和IL-10水平,苏木精—伊红(HE)染色法观察脊髓组织病理学变化,尼氏染色观察脊髓神经元细胞及尼氏小体。结果:与A组比较,B组有益菌Bacteroidetes和Saccharibacteria数量减少,BBB评分下降,脊髓组织中抗炎因子IL-10和IL-4下降和促炎因子IL-17升高(均P<0.05),炎性细胞浸润和神经元细胞核固缩,尼氏小体数量明显减少;与B组比较,C组BBB评分升高,脊髓组织IL-10和IL-4含量升高,IL-17含量下降(均P<0.05),细胞变性水肿情况改善,尼氏小体数量增加。结论:FMT疗法能够有效抑制SCI后炎症反应,从而治疗SCI。更多还原

【Abstract】 Objective: To explore the effect of fecal microbiota transplantation(FMT) therapy on rats after spinal cord injury(SCI). Methods: A total of 36 SD rats were randomly divided into control group(group A), SCI(group B), and fecal transplantation group(group C), with 12 rats in each group. Rats in groups B and C were subjected to SCI by modified Allen’s percussion method, and group A underwent sham surgery. Group C rats were given fecal bacteria solution by gavage for 14 days. Sequencing of 16 S rDNA V3-V4 highly conserved region on HiSeq platform to compare intestinal flora changes after SCI. Basso-Beattie-Bresnahan(BBB) score was used to assess the condition of the hind limbs of rats. Enzyme-linked immunosorbent assay(ELISA) was used to measure spinal cord tissues IL-17, IL-4 and IL-10 levels. The pathological changes of spinal cord tissues were observed by hematoxylin-eosin(HE) staining. The spinal cord neuronal cells and Nissler vesicles were observed by Nissler staining. Results: Compared with group A, SCI led to decrease in the number of beneficial bacteria Bacteroidetes and Saccharibacteria, BBB score, and anti-inflammatory factors IL-10 and IL-4 levels, but an increase in the proinflammatory factor IL-17 level in spinal cord tissue(all P<0.05). Inflammatory cell invasion, neuronal cell nuclear consolidation and few Nisus vesicles were found in group B. Compared with group B, the BBB score and the IL-10 and IL-4 levels in spinal cord tissues were increased, while the IL-17 level was decreased in group C(all P<0.05). Moreover, HE and Nissler staining revealed improved cellular degeneration and edema, and increased Nisus vesicles. Conclusion: Fecal bacteria transplantation therapy can effectively suppress the inflammatory response after SCI and thus treating SCI.更多还原