【摘要】 目的:探讨牡蛎葛根固体饮料(OKPD)对TritonWR-1339诱导实验性高血脂症(HLP)小鼠的血脂调节及肝脏保护作用及其机制。方法:将60只昆明小鼠(雌雄各半)随机分为正常对照组、模型组、非诺贝特阳性对照组及OKPD高、中、低剂量组,各组给予相应药物干预14 d,正常对照组和模型组给予等量生理盐水。末次给药后1 h,除正常对照组外,其他各组均腹腔注射Triton WR-1339建立HLP模型。造模24 h后,眼眶取血。检测小鼠血清中甘油三酯(TG)、总胆固醇(TC)、低密度脂蛋白胆固醇(LDL-C)、高密度脂蛋白胆固醇(HDL-C)、丙氨酸氨基转移酶(ALT)、谷草转氨酶(AST)和总一氧化氮合成酶(T-NOS)水平。采用苏木精—伊红(HE)染色法观察小鼠肝脏组织的病理改变。免疫组化法检测小鼠肝脏组织中核转录因子κB(NF-κB)、白细胞介素6(IL-6)和肿瘤坏死因子α(TNF-α)的表达。结果:与正常对照组比较,模型组小鼠血清中TC、TG、LDL-C、AST、ALT、T-NOS水平及肝组织NF-κB、TNF-α及IL-6蛋白表达显著升高,HDL-C水平显著下降(均P<0.05)。与模型组比较,OKPD高、中剂量组TC、TG、AST、ALT水平显著下降(均P<0.05);OKPD各剂量组小鼠肝脏组织细胞形态结构较模型组完整,LDL-C、T-NOS水平及肝组织NF-κB、TNF-α及IL-6蛋白表达显著下降,HDL-C水平显著升高(均P<0.05)。结论:OKPD对Triton WR-1339诱导的HLP小鼠具有降血脂及肝脏保护作用,其机制可能与减轻炎症反应有关。更多还原

【Abstract】 Objective: To investigate the effect and mechanism of oyster and kudzu root powder drink(OKPD)on blood lipid regulation and liver protection in Triton WR-1339 induced hyperlipidemias(HLP) mice. Methods:Sixty Kunming mice were randomly divided into normal control group, model group, fenofibrate positive control group, and low-, medium-, and high-dose OKPD groups. The mice were given corresponding drug for 14 days.Mice in normal control group and model group were given equal volume of normal saline. After 1 h of the last administration, except for the normal control group, the mice in other groups were intraperitoneally injected with Triton WR-1339 to establish HLP model. The levels of total cholesterol(TC), triglyceride(TG), high-density lipoprotein-cholesterol(HDL-C), low-density lipoprotein-cholesterol(LDL-C), alanine aminotransferase(ALT), aspartate aminotransferase(AST), and total nitric oxide synthase(T-NOS) in serum were detected. The pathological changes of liver tissues wereobserved by hematoxylin eosin(HE) staining. The expressions of nuclear transcription factor-kappa B(NF-κB), interleukin-6(IL-6) and tumor necrosis factor-α(TNF-α) in liver tissues were detected by immunohistochemistry. Results: Compared with the normal control group, the serum levels of TC, TG,LDL-C, AST, ALT and T-NOS, and the protein expressions of NF-κB, TNF-α and IL-6 in the model group were greatly increased, while the HDL-C level was notably decreased(P<0.05). Compared with the model group, the contents of TC, TG, AST and ALT in high-dose and medium-dose OKPD groups were evidently decreased(P<0.05). The structure of liver tissue was obviously alleviated, the serum levels of LDL-C and T-NOS and the protein expressions of NF-κB, TNF-α and IL-6 were significantly reduced, while the HDL-C concentration was markedly elevated in each dose group(P<0.05). Conclusion: OKPD exerts hypolipidemic and liver protective effects in HLP mice induced by Triton WR-1339, and the mechanism may be related to reducing inflammatory response.更多还原