【摘要】 目的:探讨miR-34a、高迁移率族蛋白B1(HMGB1)及细胞增殖核抗原(PCNA)基因在宫颈上皮内病变(SIL)组织中的表达及其与临床病理因素的关系。方法:收集广西医科大学附属肿瘤医院2016年1月至2019年12月收治的158例SIL、宫颈鳞癌(SCC)及因子宫肌瘤/子宫腺肌症切除子宫的患者宫颈组织标本及临床病理资料。将研究对象分为正常宫颈组(n=50)、SIL组(n=65)和SCC组(n=43)。采用实时荧光定量PCR(qPCR)检测与分析miR-34a、HMGB1及PCNA在组织样本和细胞系中的表达。结果:SIL组和SCC组miR-34a表达均显著低于正常宫颈组(均P<0.001),且SCC组显著低于SIL组(P=0.024)。SCC组HMGB1 mRNA水平均显著高于正常宫颈组和SIL组(P=0.001,P=0.038),且SCC组显著高于SIL组(P=0.039)。SCC组PCNA mRNA水平均显著高于正常宫颈组和SIL组(P=0.033,P=0.004),而正常组和SIL组比较,差异无统计学意义(P=0.967)。H8细胞miR-34a表达显著高于SiHa细胞,而HMGB1和PCNA的mRNA水平显著低于SiHa细胞(P<0.001,P=0.005,P=0.045)。此外,HPV阳性的SIL和SCC组织miR-34a的m RNA水平均显著下调(P=0.028,P=0.039),有淋巴结转移者miR-34a表达显著下调(P=0.015)。结论:随着宫颈病变程度的加重,miR-34a表达逐渐降低,HMGB1和PCNA表达逐渐升高,三者的变化趋势预示着SIL的进展和宫颈癌(CC)的发生,miR-34a、HMGB1及PCNA有望成为预警SIL进展的新靶标。更多还原

【Abstract】 Objective: To investigate the expression of miR-34 a, HMGB1 and PCNA genes in squamous intraepithelial lesions(SIL) and their relationship with clinicopathological factors. Methods: Cervical tissue specimens and clinicopathological information of 158 patients with SIL, cervical squamous cell carcinoma(SCC) and hysterectomy due to hysteromyoma/adenomyosis were collected. The subjects were divided into normal cervical group(n=50), SIL group(n=65) and SCC group(n=43). The expressions of miR-34 a, HMGB1 and PCNA in tissue samples and cell lines were detected and analyzed by qRT-PCR. Results: The expression of miR-34 a in SIL group and SCC group was significantly lower than that in normal group(P<0.001), and that in SCC group was significantly lower than that in SIL group(P=0.024). The mRNA level of HMGB1 in normal group and SIL group was significantly lower than that in SCC group(P=0.001, P=0.038), and that in SIL group was significantly lower than that in SCC group(P=0.039). The mRNA levels of PCNA in normal group and SIL group were significantly lower than those in SCC group(P=0.033, P=0.004), but there was no significant difference between normal groupand SIL group(P=0.967). The expression of mi R-34 a in H8 cells was significantly higher than that in Si Ha cells, while the m RNA levels of HMGB1 and PCNA in H8 cells were significantly lower than those in SiHa cells(P<0.001, P=0.005, P=0.045). In addition,the m RNA levels of mi R-34 a in HPV positive SIL and SCC were significantly down regulated(P=0.028, P=0.039), and the expression of mi R-34 a in patients with lymph node metastasis was significantly down regulated(P=0.015). Conclusion: With the aggravation of cervical lesions, the expression of mi R-34 a gradually decreases,while the expression of HMGB1 and PCNA gradually increases. The change trend of the three indicates the progress of SIL and the occurrence of cervical cancer. Mi R-34 a, HMGB1 and PCNA are expected to become new targets for early warning the progress of SIL.更多还原