【摘要】 采用同步荧光技术结合化学计量学实现了鸡肉中磺胺二甲基嘧啶(Sulfamethazine,SM2)和氧氟沙星(Ofloxacin,OFL)残留的快速检测。分析了SM2标准溶液、OFL标准溶液、空白鸡肉提取液和含SM2和OFL的鸡肉提取液的三维同步荧光光谱,确定了鸡肉中SM2和OFL残留检测的波长差(Δλ)分别为150 nm和210 nm,荧光激发峰分别为292.5nm和295 nm。采用单因素实验考察了β-巯基乙醇和邻苯二甲醛溶液加入量及时间对荧光强度的影响,确定了最佳检测条件为:β-巯基乙醇溶液300μL、邻苯二甲醛溶液25μL和采集时间44 min。最后,利用峰高法和峰面积法分别建立了预测模型。实验结果表明,与基于峰面积法的预测模型相比,基于峰高法的预测模型的综合评价更好。基于峰高法的SM2和OFL残留预测模型的预测集决定系数(R_P~2)分别为0.897 3和0.997 3,预测集均方根误差(RMSEP)分别为6.060 5 mg/kg和0.539 2 mg/kg,回收率分别处于76.1%～115.2%和96.7%～110.1%之间,相对标准偏差(RSD)分别处于2.7%～7.0%和2.8%～10.0%之间。本方法快速、简便,可用于鸡肉中SM2和OFL残留的快速检测。更多还原

【Abstract】 Rapid detection of sulfamethazine (SM2) and ofloxacin (OFL) residues in chicken was achieved through synchronous fluorescence technology coupled with chemometric methods.First,the three-dimensional synchronous fluorescence spectra of an SM2 standard solution,an OFL standard solution,chicken extract without antibiotics,and chicken extract containing SM2 and OFL residues were analyzed.The wavelength difference(Δλ)of SM2 and OFL were determined to be 150 nm and 210 nm,respectively.The fluorescence excitation peaks of SM2 and OFL were determined to be 292.5 nm and 295nm,respectively,for the detection of SM2 and OFL residues in chicken.Subsequently,the effects of addition amounts ofβ-mercaptoethanol solution and o-phthalaldehyde solution,as well as time,on the fluorescence intensities were investigated through the single factor test.Aβ-mercaptoethanol solution of 300μL,an o-phthalaldehyde solution of 25μL and a time of 44 min were the best combination of conditions for detections.Finally,the prediction models were established by the peak height and peak area algorithms respectively.The experimental results show that the prediction model based on the peak height algorithm provides a better comprehensive evaluation than the prediction model based on the peak area algorithm.For the prediction models of SM2 and OFL residues based on the peak height algorithm,the coefficients of determination for the prediction set(R_P~2) are 0.897 3 and 0.997 3 for SM2 and OFL,respectively.The root mean square errors for the prediction set(RMSEP)are 6.060 5 mg/kg and 0.539 2 mg/kg,for SM2 and OFL,respectively.The recovery rates of SM2 and OFL are in the range of 76.1%-115.2% and 96.7%-110.1%,respectively,and the relative standard deviations(RSDs)are in the range of 2.7%-7.0%and 2.8%-10.0%,respectively.The proposed method is fast and simple and could achieve rapid detection of SM2 and OFL residues in chicken.更多还原