【摘要】 为了实现胰岛素对糖尿病患者血糖浓度智能调控,提出以3-丙烯酰胺基苯硼酸（AAPBA）、N,N’-二甲基丙烯酰胺（DMAA）、丙烯酰胺（AAm）为单体,70℃下经乳液聚合制备Poly（AAPBA-DMAA-co-AAm）微凝胶。以微凝胶为交联点,氧化石墨烯（GO）为改进剂,制备了GO/微凝胶复合水凝胶。采用傅里叶红外光谱仪（FT-IR）,X射线衍谢仪（XRD）和扫描电镜（SEM）对水凝胶的组成、表面形貌进行表征,粒径分析表明微凝胶的粒径分布均匀、分散性良好。通过称重法对水凝胶的葡萄糖响应性进行测试,结果表明在p H为7.3条件下,水凝胶的响应速率随着葡萄糖浓度的增加而增大。当葡萄糖浓度为40 mmol×L^-1,GO/微凝胶复合水凝胶对葡萄糖最大响应溶胀度为18%,响应时间为30 min,载药水凝胶的体外释放结果表明,10 h胰岛素累计释放率可达到52.59%,GO/微凝胶复合水凝胶对胰岛素具有缓释控释作用。更多还原

【Abstract】 In order to control blood glucose levels of diabetic patients using insulin, poly(AAPBA-DMAAco-AAm) microgels were fabricated via emulsion polymerization of dimethyl acryl amide(DMAA), acryl amide(AAm) and 3-acrylamidophenylboronic acid(AAPBA) at 70 ℃. GO/Nanocomposite hydrogels were prepared using microgels as micro-crosslinkers and graphene oxide(GO) as an additive, and their composition and surface morphology were characterized by FT-IR, XRD and SEM. The particle size distribution of the microgels observed by particle size analyzer was uniform and dispersive. The response rate of hydrogels increased along with the increase of glucose concentration at pH=7.3 by weighing. When the glucose concentration was 40 mmol×L-1 and the GO additive amount was 1.0%, the highest swelling degree of GO/nanocomposite hydrogels was 18% and the response time was 30 min. The in vitro study of insulin release indicated that the accumulated release amount of insulin reached 52.59% within 10 h, and GO/nanocomposite hydrogels exhibited better sustained-release ability.更多还原