【摘要】 目的建立三叶海棠叶中单一成分2-(（4-（3,4-二羟基苯基）-2-氧代丁氧基-甲氧基）-2-羟基丙二酸-1-β-D-吡喃葡萄糖单酯的含量测定方法。方法采用高效液相色谱法测定,色谱柱为Phenomenex C₁₈（4.6 mm×150 mm,5μm）,流动相为乙腈-水（21∶79）,流速为1.0 ml/min,检测波长为285 nm;柱温为40℃;进样量为20μl。结果 PMAGE在4.5～180.0μg/ml的范围内具有良好的线性关系（r=0.9999）,三叶海棠叶中PMGAE的平均含量为8.12%,平均加样回收率为100.6%,RSD为1.7%（n=5）。结论三叶海棠叶中PMAGE的含量较高,本测定方法灵敏度高、结果准确、重现性好,可用于三叶海棠叶中PMAGE的含量测定。更多还原

【Abstract】 Objective To establish a method for the determination of 2-(（ 4-（ 3, 4-dihydroxyphenyl）-2-oxobutoxy methoxy）-2-hydroxymalonic acid-1-β-D-glucopyranose monoester in leaf of Malus sieboldji. Methods High performance liquid chromatography was used in the determination with Phenomenex C₁₈ column（ 4. 6 mm × 150 mm,5 μm）. The chromatographic conditions were as follows:acetonitrile-water（ 21∶ 79） as mobile phase,the volumn flow rate was 1. 0 ml/min,the wavelength was detected at 285 nm,the column temperature was 40℃,and the injection volume was 20 μl. Results The content of PMAGE had a good linear relationship in the range of 4. 5 ～ 180. 0 μg/ml,with the value of 0. 9999,and the linear equation was Y = 4. 6 × 10⁴ X + 1. 69 × 10⁵ in the range of 4. 5 ～ 180. 0 μg/ml.The average content of PMAGE in leaf of Malus sieboldii was 8. 12%,and the average recovery of PMAGE was 100. 6% with RSD 1. 7%（ n = 5）. Conclusion The method is sensitive,accurate reliable and repeatable for determination of PMAGE.更多还原