【摘要】 以福白菊为原料,通过有机溶剂萃取和水蒸气蒸馏相结合制备福白菊精油.分别采用健那绿、细胞膜荧光探针DiI、碘化丙啶、罗丹明123以及溴化乙锭等染料标记酵母细胞、酵母细胞膜、酵母线粒体和质粒DNA,研究福白菊精油对酵母细胞、酵母细胞膜、线粒体以及质粒DNA的破坏作用,探讨福白菊精油的抗真菌机理.研究结果表明,福白菊精油不仅能破坏真菌的细胞膜,使其细胞内含物外泄,还能够穿过细胞膜,破坏其线粒体膜和DNA,最终导致真菌细胞死亡.更多还原

【Abstract】 Fubaiju essential oil was prepared by organic solvent extraction and steam distillation method.The cells,cell membrane,nucleic acid,mitochondrion and circular plasmid pCAMBIA1303 of S.cerevisiae were labeled with Jianna green,1,1’-dioctadecyl-3,3,3’,3’-tetramethylindocarbocyanine perchlorate,propidium iodide,rhodamine 123 and ethidium bromide respectively to investigate antifungal mechanism of Fubaijuessential oil.According to the results of fluorescence microscope,Fubaiju essential oil showed antifungal activity against S.cerevisiae.Fubaiju essential oil could not only destroy the cell membrane,make the cell contents leak out,but also penetrate the cell membrane,destroy the mitochondrial membrane and DNA,and eventually lead to cell death.更多还原