【摘要】 设计合成了对称型菲并咪唑荧光探针PIP-ph-PIP,并对其结构进行了表征和确认.随着探针溶液中水体积分数的增加,探针的聚集程度逐渐增加,荧光强度先增强后猝灭.荧光光谱测试结果表明,在水相体系中探针PIP-ph-PIP能以ON-OFF-ON的方式分别对Ag⁺和SCN^-,Cu²⁺和PO₄^3-进行连续识别,且连续识别效果可通过裸眼比色观察,其中对Ag⁺识别具有超低检出限（6.1 nmol/L）.结果表明,探针PIP-ph-PIP可应用于活体He La细胞中Ag⁺和Cu²⁺的定性分析及实际水样中Ag⁺和Cu²⁺的定量检测.更多还原

【Abstract】 The symmetric fluorescent probe PIP-ph-PIP based on phenanthro[9,10-d]imidazole was designed and synthesized,and its structure was characterized and confirmed.As the increasing amount of water in the solution of PIP-ph-PIP,the fluorescence intensity of PIP-ph-PIP first increased and then quenched with the gradual aggregation.The results of the fluorescence spectra indicated that the probe PIP-ph-PIP could selectively and successively recognize Ag⁺and SCN^-，Cu²⁺and PO₄^3-，respectively,with an ON-OFF-ON mode in the aqueous system.Moreover,the continuous effect can be observed by the colorimetric naked eye.The probe PIP-ph-PIP offered a relatively low detection limits of 6.1 nmol/L for Ag⁺ions.The probe PIP-ph-PIP was successfully applied to detect Ag⁺and Cu²⁺in living human cancer cells（He La cells）and quantitively analyze of Ag⁺and Cu²⁺in actual water samples.更多还原