【摘要】 从薤(Allium chinense G. Don)的乙醇提取物中分离得到6个新甾体皂苷类化合物,通过波谱数据及理化性质分析,鉴定其分别为5α-cholano-22,16-内酯-3-O-β-D-吡喃葡萄糖基-(1→2)-[β-D-吡喃葡萄糖基-(1→3)]-β-D-吡喃葡萄糖基(1→4)-β-D-吡喃半乳糖苷(1)、6-酮-5α-cholano-22,16-内酯-3-O-β-D-吡喃木糖基-(1→4)-[α-L-吡喃阿拉伯糖基-(1→6)]-β-D-吡喃葡萄糖苷(2)、(25R)-26-O-β-D-吡喃葡萄糖基-5α-呋喃甾烷-3β,26-二醇-3-O-β-D-吡喃葡萄糖基-(1→2)-[β-D-吡喃葡萄糖基-(1→3)]-β-D-吡喃葡萄糖基(1→4)-β-D-吡喃半乳糖苷(3)、(25R)-6-酮-26-O-β-D-吡喃葡萄糖基-5α-呋喃甾烷-3β,22α,26-三醇-3-O-α-L-吡喃木糖基-(1→4)-β-D-吡喃葡萄糖苷(4)、(25R)-6-酮-5α-呋喃甾烷-3β,22α,24β,26-四醇-3-O-β-D-吡喃木糖基-(1→4)-[α-L-吡喃阿拉伯糖基-(1→6)]-β-D-吡喃葡萄糖苷(5)和(25R)-5α-呋甾-2α,3β,22α, 26-四醇-26-O-β-D-吡喃葡萄糖苷(6).化合物1和2的皂苷元骨架在天然产物中首次分离得到.选用H2O2诱导PC12细胞神经氧化损伤模型,初步考察了6种新的呋甾型化合物的抗氧化活性,实验结果表明,化合物3对由H2O2诱导的细胞氧化损伤有显著的保护效果.更多还原

【Abstract】 Six new steroidal saponins were isolated from 98% ethanol extract of Allium chinense G. Don.Through spectral data as well as physical and chemical analysis,they were identified as 5α-cholano-22,16-lactone-3-O-β-D-glucopyranosyl-(1→2)-[β-D-glucopyranosyl-(1→3)]-β-D-glucopyranosyl-(1→4)-β-D-galacopyranoside(1),6-ketone-5α-cholano-22,16-lactone-3-O-β-D-6-xylopyranosyl-(1→4)-[α-L-arabinopyranosyl-(1→6)]-β-D-glucopyranoside(2),(25R)-26-O-β-D-glucopyranosyl-5α-furostane-3β,26-diol-3-O-β-Dglucopyranosyl-(1→2)-[β-D-glucopyranosyl-(1→3)]-β-D-glucopyranosyl-(1→4)-β-D-galacopyranoside(3),(25R)-6-ketone-26-O-β-D-glucopyranosyl-5α-furostane-3β,22α,26-triol-3-O-α-L-xylopyranosyl-(1→4)-β-D-glucopyranoside(4),(25R)-6-ketone-5α-furostane-3β,22α,24β,26-tetraol-3-O-β-D-xylopyranosyl-(1→4)-[α-L-arabinopyranosyl-(1→6)]-β-D-glucopyranoside(5)and(25R)-5α-furostane-2α,3β,22α,26-tetraol-26-O-β-D-glucopyranoside(6). To the best of our knowledges,the sapogenin skeletons of compound 1 and compound 2 were isolated from natural products for the first time. The cell proliferation-toxicity test kit(CCK-8)was used to explore the PC12 cells hydrogen peroxide induced the protective effect of oxidative damage. The experimental results showed that compound 3 had a significant protective effect on cell oxidative damage induced by hydrogen peroxide.更多还原