【摘要】 用体外药敏试验评价了丁香酚抗马拉色菌的活性,并通过棋盘微量稀释法研究了丁香酚与传统去屑剂联合用药的相互作用,丁香酚的最低抑菌浓度(Minimum Inhibitory Concentration, MIC)为312.50μg/m L,最低杀菌浓度(Minimum Fungicidal Concentration, MFC)为625.00μg/m L;与酮康唑联用表现为协同作用,与吡啶硫酮锌(Zinc Pyrithione, ZPT)、氯咪巴唑、吡啶酮乙醇胺盐(Piroctone Olamine, OCT)分别联用表现为相加作用;用四甲基偶唑盐(3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromid,MTT)法评价了丁香酚对角质形成细胞的毒性,其半抑制浓度(50%Inhibitory Concentration,IC50)为320.93μg/m L,高于传统去屑剂;最后通过扫描电子显微镜(Scanning Electron Microscope, SEM)和透射电子显微镜(Transmission Electron Microscope, TEM)初步研究了丁香酚对马拉色菌的作用机制,结果表明丁香酚通过破坏细胞壁和细胞内部膜结构达到杀菌的效果。更多还原

【Abstract】 The in vitro susceptibility test was used to evaluate the activity of eugenol against Malassezia, and the interaction between eugenol and traditional antidandruff drugs was studied by the checkerboard microdilution method. The minimum inhibitory concentration(MIC) of eugenol was 312.50 μg/m L and the minimum fungicidal concentration was 625.00 μg/m L. Eugenol combined with ketoconazole showed a synergistic effect, and combined with zinc pyrithione(ZPT), climbazole, and piroctone olamine(OCT) respectively showed additive effects.Combined use can reduce the minimum effective concentration of each drug by 50%～75%. The toxicity of eugenol to keratinocytes was evaluated by MTT(3-(4, 5-Dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromid) assay. The results showed that the IC50 of eugenol was 320.93 mg/L, which is higher than that of traditional antidandruff agents. This indicated that eugenol has higher safety at the cellular level. The mechanism of eugenol against Malassezia was studied by Scanning Electron Microscopy(SEM) and Transmission Electron Microscopy(TEM).It was found that eugenol achieves fungicidal ability by destroying cell wall and internal membrane structure of Malassezia. These results provided a theoretical basis for the application of eugenol in antidandruff products.更多还原