【摘要】 【目的】探明干旱胁迫下花生转录组与miRNA测序及相关基因的表达,为深入分析花生抗旱分子机制提供理论依据。【方法】采用转录组测序和miRNA测序技术,对桂花37花生在15%PEG6000模拟干旱胁迫下基因和miRNA的表达模式进行分析。【结果】转录组测序发现有13个显著持续上调的差异基因,其中7个为HSP分子伴侣家族基因,2个RING E3泛素连接酶,1个为谷胱甘肽-S-转移酶,1个柱头特异性STIG1样蛋白1,1个免疫球蛋白结合蛋白和1个DnaJ同源B亚家族成员13;同时筛选出13个显著下调的差异基因;在miRNA测序数据中筛选到8个持续下调表达的miRNA,分别ahy-mir156-3p、ahy-miR159、ahy-miR167-5p、ahy-miR156a、ahy-miR156b-5p、novel_mir103、novel_mir83和novel_mir70。【结论】持续下调表达的miRNA通过负调控方式参与花生干旱胁迫过程。更多还原

【Abstract】 【Objective】Transcriptome and miRNA sequencing of peanut and related genes expression under drought stress is explored,which provide the theoretical basis for deeply analyzing the drought-resistance molecule mechanism of peanut.【Method】The expression patterns of gene and miRNA in peanut cultivar Guihua 37 under 15% PEG6000 stimulated drought stress are analyzed by using transcriptome sequencing and miRNA sequencing technique.【Result】A total of 13 significantly and continuously up-regulated differential genes have been found by transcriptome sequencing,including seven HSP molecular chaperone family genes,two RING E3 ubiquitin ligases,one glutathione S-transferase,one stigma-specific STIG1-like protein 1,one immunoglobulin binding protein and one DnaJ homologous B subfamily member 13.A total of 13 significantly down-regulated differential genes are screened out at the same time.Eight continuously down-regulated miRNAs,including ahy-mir156-3p,ahy-miR159,ahy-miR167-5p,ahy-miR156a,ahy-miR156b-5p,novel_mir103,novel_mir83 and novel_mir70,are screened out based on miRNA sequencing data.【Conclusion】Continuously down-regulated miRNA involves the drought stress process of peanut through negative regulation pattern.更多还原