【摘要】 试验采用超高效液相色谱法（UPLC）对浓缩饲料中维生素K₃的含量进行测试,并评估此试验的测量不确定度。浓缩饲料中的维生素K₃类饲料添加剂经三氯甲烷和碳酸钠溶液提取,转化为游离的甲萘醌,经C₁₈反相色谱柱分离,PDA检测器在251nm波长处检测,外标法定量即得浓缩饲料中维生素K₃的含量。经检测浓缩饲料中维生素K₃的含量为:（1.42±0.07）mg·kg^-1,k=2（95%置信度）。测量不确定度的评定结果表明,标准储备液和标准工作液配制过程是影响测量结果的主要因素。更多还原

【Abstract】 Vitamin K₃ was extracted by chloroform and sodium carbonate solution and then transformed into free menadione.It was separated by C₁₈ reversed-phase chromatographic column.The vitamin K₃ was determined by PDA detector at the wavelength of 251 nm,and quantified by external standard method.The test results showed that the content of vitamin K₃ was（1.42±0.07）mg·kg^-1,k=2（95%confidence）.The evaluation results showed that the main influence factors were the preparation process of standard stock solution and working solution.更多还原