【摘要】 采用单因素试验和Box-Behnken试验结合,研究了液料比、柠檬酸浓度、提取时间对花青素提取率的影响,以花青素提取率为响应值,确定了其最佳提取工艺条件是:液料比25:1(m L/g),柠檬酸浓度10.5%,提取时间29 min,提取率为(5.9±0.89)%。基于此,用AB-8大孔树脂纯化花青素粗提液初步纯化以除去多糖杂质,结果表明,纯化条件为上样量为1 BV,洗脱液为75%乙醇用量3～6 BV。纯化后花青素粗提液多糖去除率达(99.3±0.972)%,纯化后提取物中花青素含量为(39.984±1.24)mg/g,本研究结果可为紫薯花青素的提取与纯化提供一定的参考。更多还原

【Abstract】 In this paper, the effects of ratio of liquid-material citric acid concentration and extraction time on the extraction rate of anthocyanins are studied by single factor test and Box Behnken test. According to the response value of anthocyanin extraction rate, the optimum extraction conditions were liquid material ratio 25:1(m L/g),citric acid concentration 10.5%, extraction time 29 mins and extraction rate(5.9 ±0.89)%. Therefore, the anthocyanin crude extract was preliminarily purified with AB-8 macroporous resin to remove polysaccharide impurities. The results showed that the purification condition was 1 BV and elution condition was 75% ethanol3～6 BV. After purification, the removal rate of polysaccharide in the crude extract of anthocyanin was(99.3±0.972)%, and the content of anthocyanin in the purified extract was(39.984±1.24)mg/g. The results of this study can provide some reference for the extraction and purification of anthocyanin from purple potato.更多还原