【摘要】 为研究日本囊对虾新品种的选育新技术,试验设置50、100、1 000、1 500 cGy ⁶⁰Co-γ射线四种照射剂量,对即将产卵的日本囊对虾进行照射;在照射强度为3.4 cGy/sec的条件下,采用AFLP分子标记技术从分子水平对各组诱变子代的遗传相似系数、遗传距离以及遗传多样性影响等差异进行分析;统计了日本囊对虾诱变子代在室外池塘中生长的体质量数据;单因素方差分析检测其差异显著性。实验结果表明,日本囊对虾诱变子代遗传相似系数在0.873 7～0.894 7之间;从群体水平来看,日本囊对虾诱变子代与对照组相比（P>0.05）,并没有显著的遗传变异出现;在诱变的实验组中发现有单个个体的基因型与其他个体的基因型相比,具有较为明显的差异,而在对照组中并没有发现类似个体的存在;池塘中各实验组和对照组的生长情况具有较为显著的差异。本研究结果可为日本囊对虾的诱变育种工作提供基础数据和科学依据。更多还原

【Abstract】 The present study conducted four different radiation dose of 50 cGy, 100 cGy, 1 000 cGy and 1 500 cGy in order to research the new kind breeding of Marsupenaeus japonicus.In our study, the ⁶⁰Co-γ radiation was conducted on the condition of 3.4 cGy/sec with M.japonicus which was about to spawn.We applied the AFLP molecular technology to analyze the genetic similarities, genetic distances and genetic diversity of the ⁶⁰Co-γ-radiated progeny in each group.The weight data of M.japonicus progeny in each group grown in earth ponds over 90 days were also counted to supply the proof for the radiation breeding programme of M.japonicus.All those experimental data were conducted with single factor analysis of variance to test the difference.The experiment results indicated that the genetic similarities was from 0.873 7 to 0.894 7 and the genetic diversity of ⁶⁰Co-γ-radiated progeny was not significant different from the control group in populations（P>0.05）.But we found some obvious distinctions in the genome type of single individual in ⁶⁰Co-γ-radiated groups compared to others which did not exist in control group.Meanwhile, the growth in earth ponds was significant different in each group.Our research provided fundamental data and science proof for the radiation breeding programme of M.japonicus.更多还原