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紫红曲霉鉴定及两阶段培养产多糖条件优化

Identification of Monascus Purpureus and Optimization of the Conditions for Polysaccharide Production in the Two-stage Culture

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【作者】 余慧菁蒋咏梅章文贤

【Author】 YU Hui-jing;JIANG Yong-mei;ZHANG Wen-xian;College of Life Science,Fujian Normal University;Engineering Research Center of Ministry of Education of Industrial Microbiology;

【通讯作者】 章文贤;

【机构】 福建师范大学生命科学学院工业微生物教育部工程研究中心

【摘要】 采用形态学特征及ITS序列分析对已筛选的菌株进行鉴定。为优化振荡-静置两阶段培养红曲霉产胞内多糖的条件,分别测定了摇瓶过程中菌丝球数量、发酵液黏度、pH和残糖含量,以及静置培养中菌丝体生物量、内多糖的含量和产量。结果表明:紫红曲霉在振荡培养3d后再静置培养6d的条件下,菌丝球大小适中,生物量较高,发酵液黏度低,传质效果好,pH中性,红曲霉内多糖含量和产量均达到最高值,分别为18.9mg·g-1和138.9mg·L-1

【Abstract】 The morphological characteristics and ITS sequence analysis were used to identify the screened strains.In order to optimize the conditions for the production of intracellular polysaccharides from Monascusin the two stages of oscillating and static culture,the number of mycelium pellets,the viscosity of fermentation broth,pH and the content of residual sugar during the process of shake flask were determined,as well as the biomass of mycelia,the content and yield of intracellular polysaccharides in the static culture.The results showed that under the conditions of oscillating culture for 3dand then static culture for 6d,Monascus purpureus had moderate size of mycelium pellets,high biomass,low viscosity of fermentation broth,good mass transfer effect and neutral pH.The content and yield of intracellular polysaccharides in Monascus reached the highest values,which were 18.9mg·g-1and 138.9mg·g-1,respectively.

【基金】 福建省自然科学基金项目(2017J01624)
  • 【文献出处】 福建农业科技 ,Fujian Agricultural Science and Technology , 编辑部邮箱 ,2021年12期
  • 【分类号】TQ929.2
  • 【下载频次】176
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