【摘要】 β-淀粉酶（BAM）家族是催化植物淀粉转化为麦芽糖的重要酶,在调控植物生长发育和非生物胁迫耐受过程中有重要作用。为了解锥栗ChBAM基因家族的结构和表达特征,对ChBAM进行了全基因组鉴定和进化分析,并研究了ChBAM在不同组织、果实发育和种子萌发期间的表达模式。结果表明,鉴定出9个ChBAM基因,均具有典型的Glyco_hydro₁4结构域,分为4个组（Ⅰ～Ⅳ组）,与其他物种比较,ChBAM家族成员和枳CtBAM在进化关系上更为接近。葡糖基水解酶结构域、基因结构和基序分析表明ChBAM家族基因高度保守。3个ChBAM蛋白与α-淀粉酶（AMY）、4-α-葡聚糖转移酶（DPE）和淀粉异构酶（ISA）等蛋白互作。ChB AM基因启动子顺式作用元件分析表明其参与了多种非生物胁迫。锥栗ChB AM家族基因具有明显的组织表达差异性,ChB AM1/9在花、叶柄、壳斗部位表达较高。在果实发育和种子萌发阶段,ChB AM1/6/9表达较高,表明ChB AM1/6/9可能在锥栗果实淀粉代谢过程发挥作用。更多还原

【Abstract】 The β-amylase（BAM） gene family encode enzymes that catalyze the conversion of starch to maltose in plants, playing an important role in regulating plant growth and development and abiotic stress tolerance. In order to understand the structure and expression characteristics of ChBAM in Castanea henryi, genome-wide identification and the evolutionary analysis of ChBAM were conducted, and the expression patterns of ChBAM in different tissues during fruit development and seed germination were studied. The results identified 9 ChBAM genes with a typical Glyco_hydro₁4 domain, which were divided into 4 groups（Groups Ⅰ-Ⅳ）. Compared with other species, the ChBAM family members are more evolutionarily closely related to the Citrus trifoliata CtBAM members. The glucosyl hydrolase domain alignment, gene structure, and motif analysis indicated that ChBAM gene family was highly conserved. Three ChBAM proteins interacted with α-amylase（AMY）, 4-α-glucan transferase（DPE）, and starch isoamylase（ISA）, etc. The cis-acting elements resulting from the ChBAM gene promoter analysis indicated that the ChBAM genes are involved in various abiotic stresses. The ChBAM family genes of Castanea henryi had obvious expression differences in tissues, and the expression of ChBAM 1/9 was higher in the flower, petiole, and cupule. The expression of ChBAM 1/6/9 was higher during fruit development and seed germination, suggesting that ChBAM 1/6/9 might play a role in starch metabolism in Castanea henryi fruit.更多还原