【摘要】 应用具有高效嘌呤核苷降解能力的乳酸菌进行高尿酸血症(HUA)辅助治疗和预防,可以减轻或解除饮食限制对HUA患者生活质量的影响。采用高效液相色谱法对从泡菜中分离得到的56株乳酸菌的嘌呤核苷降解能力进行测定,获得一株具有高效嘌呤核苷降解能力的乳酸菌,其肌苷、鸟苷的降解率可达98.07%和96.1%。经生化鉴定实验和16S rRNA序列扩增和比对,确定该菌为短乳杆菌(Laclobacillus brevis)。耐受性实验表明:该菌具有良好的耐酸、耐胆盐以及耐受饱腹人工胃肠液的能力,且其在饱腹的人工肠液中嘌呤核苷降解能力不受影响。因此,该菌株是可用于HUA防治微生态菌剂开发的潜在优良菌株。更多还原

【Abstract】 Adjunctive treatment and prevention of hyperuricemia(HUA) by lactic acid bacteria(LAB) with high purine nucleoside degrading capacity enable relieving or avoiding the impact of dietary restriction on HUA patients. In this study, the degradation capacity of purine nucleoside of 56 lactic acid bacteria isolated from kimchi was determined by high performance liquid chromatography(HPLC) analysis, which resulted a lactobacillus with highly effective purine nucleoside degradation ability. Its degradation rates of inosine and guanosine were 98.07% and 96.1%, respectively. Based on biochemical testing and 16 S rDNA classification, the strain was identified as Laclobacillus brevis. The bacterium showed excellent tolerance capacity in the condition of acid, bile salt and artificial intestinal fluid with food suspension, and its nucleoside-decomposing ability was not affected by the environment of artificial intestinal juice with food suspension. Therefore, this strain is a potential medicinal strain that can be used for developing microecological agent to prevent and treat HUA.更多还原