【摘要】 目的探讨神经精神性狼疮(NPSLE)患者血清中血小板反应蛋白-1(TSP-1)的表达及临床意义。方法 53例风湿疾病血液标本取自郑州大学第五附属医院风湿免疫科2016年1月至2020年1月住院患者,NPSLE组34例,SLE组19例,健康对照组17例,应用ELISA法检测血清中TSP-1表达水平。分析NPSLE组、SLE组与健康对照组TSP-1蛋白表达量的差异。分析TSP-1蛋白表达量与NPSLE患者血细胞计数、补体C3、补体C4、血沉、免疫球蛋白、系统性红斑狼疮疾病活动度评分(SLEDAI)等指标的相关性。结果健康对照组血清中TSP-1的水平显著高于NPSLE组及SLE组(P <0.05),TSP-1在红细胞减少(P <0.05)、血红蛋白减少(P <0.05)、抗核小体抗体阳性(P <0.05)的NPSLE患者中表达显著下降,TSP-1与红细胞(r=0.456,P=0.007)、血红蛋白(r=0.468,P=0.005)、血小板(r=0.508,P=0.002)、补体C3(r=0.301,P=0.048)呈正相关。结论 TSP-1可能在NPSLE疾病过程中发挥免疫负调节作用,对NPSLE的诊断具有一定意义。更多还原

【Abstract】 Objective To investigate the expression and significance of thrombospondin-1(TSP-1) in the serum of patients with neuropsychiatric systemic lupus erythematosus(NPSLE).Methods Blood samples of 53 patients with rheumatic diseases were collected from the Department of rheumatic immunology,the Fifth Affiliated Hospital of Zhengzhou University from January 2016 to January 2020. A total of 34 NPSLE patients,19 SLE patients and 17 healthy controls with matched age and gender were included in this study. The serum TSP-1 level was detected by ELISA. The differences of TSP-1 expression levels among NPSLE group,SLE group and healthy control group were analyzed. The relationship between TSP-1 and blood cell count, complement 3, complement 4, erythrocyte sedimentation rate,immunoglobulin,SLEDAI and other indicators were analyzed. Results The level of TSP-1 in the serum of the healthy control group was significantly higher than that of the NPSLE group and the SLE group(P < 0. 05). A further significant decrease of serum TSP-1 was still observed in the patients with erythrocyte reduction(P < 0. 05), hemoglobin reduction(P < 0. 05), and positive antinucleosome antibody(P < 0. 05). TSP-1 serum levels in NPSLE patients was positively correlated with red blood cells(r = 0. 456,P = 0. 007),hemoglobin(r = 0. 468,P = 0. 005),platelets(r = 0. 508,P = 0. 002),Complement 3(r = 0. 301,P = 0. 048). Conclusions TSP-1 may play a negative immunoregulatory role in the development of NPSLE,which had promising value in the diagnosis of neuropsychiatric systemic lupus erythematosus.更多还原