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磷钨酸和干燥处理提高植物样品显微CT成像对比度的方法

New method of improving micro-CT images contrasts on plant samples

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【作者】 徐秀苹孟淑春梁荣花靳婉青冯旻

【Author】 XU Xiu-ping;MENG Shu-chun;LIANG Rong-hua;JIN Wan-qing;FENG Min;State Key Laboratory of Systematic and Evolutionary Botany, Institute of Botany, Chinese Academy of Sciences;Plant Science Facility of the Institute of Botany, Chinese Academy of Sciences;Beijing Vegetable Research Center, Beijing Academy of Agricultue and Forestry;

【通讯作者】 冯旻;

【机构】 中国科学院植物研究所系统与进化植物学国家重点实验室中国科学院植物研究所公共技术服务中心北京市农林科学院蔬菜研究中心

【摘要】 显微CT技术是一种非破坏性的三维成像技术,在医学、地质学、材料学等方面有着广泛的应用,在植物成像方面也是一种前景很好的技术手段。但植物组织主要由轻元素构成,X射线成像对比度很低,其应用受到很大限制。本文在前人的研究基础上,使用不同浓度的磷钨酸处理不同植物样品,比较不同处理时间、不同植物结构的差异,经CO2临界点干燥后再扫描等前处理方法对显微CT成像效果的影响,探索出增强植物材料成像对比度的处理流程,使其更适用于显微CT扫描。

【Abstract】 Micro-CT technology is a nondestructive three-dimensional imaging technology and has played a great role in researches on medicine, geology, materials science and other fields. It is also a very promising technique in plant micro-imaging. However, due to the specially lighter element composition of the plant samples, the application in plants is still limited. In order to improve the images contrasts of micro-CT of plant samples, different plant materials were treated by phosphotungstic acid with different concentrations and time, plus dried by CO2 critical point drying, then scanned by micro-CT and their images are compared. The results show that new methods can enhance the image contrasts of plant materials and make it much more suitable for micro-CT scanning.

【基金】 中国科学院仪器设备功能开发技术创新项目(No.Y7111G1001)
  • 【文献出处】 电子显微学报 ,Journal of Chinese Electron Microscopy Society , 编辑部邮箱 ,2021年04期
  • 【分类号】Q94-33
  • 【下载频次】137
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