【摘要】 出芽酵母已经成为衰老、寿命研究的理想细胞模型。我们提出了一种集成微电极阵列微流控芯片的设计结构,该芯片结构具有阵列排布式捕获-剪切结构及与之对应的微电极阵列,用于对被捕获酵母单细胞进行高通量电阻抗检测,尤其是利用电阻抗信号检测酵母细胞每一个子细胞的剪切去除事件。我们对该设计结构进行了有限元建模仿真研究,以优化用于电阻抗检测的集成微电极阵列的关键参数设置。通过有限元建模和仿真计算,我们分析了待测响应电流分布以及不同行列间距下邻近细胞对待测信号的影响。为了在减小邻近细胞的存在对待测信号干扰的同时,实现酵母子细胞剪切去除事件的高灵敏度电阻抗检测,我们依据仿真结果优化出微电极阵列的行列间距分别为125μm、100μm。本研究中的仿真分析结果对于优化集成微电极阵列微流控芯片的设计,提升电阻抗单细胞传感检测的灵敏度和集成度具有重要意义,我们提出的设计结构有望发展为基于电阻抗谱的高通量酵母复制衰老寿命监测平台。更多还原

【Abstract】 Budding yeast(Saccharomyces cerevisiae)has become an ideal cell model for the study of aging and longevity. We proposed a design of a microelectrode-array-integrated microfluidic device, which features a capture-dissection array and a corresponding microelectrode array to perform high-throughput electrical impedance measurements of immobilized single yeast cells, especially for the detection of dissection events of yeast daughter cells through electrical impedance spectroscopy(EIS). In this work, we investigated the device through finite element modeling and simulation to optimize the design parameter of microelectrode array for EIS measurements. The distribution of recording current and the influence of adjacent cells on the simulated EIS signals at different row and column spacing were analyzed through FEM simulation and numerical calculation. To reduce disturbance of adjacent cells on the EIS signals and ensure a high sensitive EIS detection of dissection events of yeast daughter cells, the row and column spacing were optimized to be 125 μm and 100 μm respectively based on the simulation results. Therefore, the simulation results are of vital significance to optimize the design of a microelectrode-array-integrated microfluidic device and improve the sensitivity and throughput of single-cell EIS sensing and measurements. Moreover, the proposed design can be potentially developed to be a high-throughput EIS-based platform for the determination of yeast replicative lifespan(RLS).更多还原