【摘要】 为研究N⁺离子注入黑花生对Oligomeric Proaflth Cyaflidin （OPC）清除氧自由基能力的影响,将不同能量剂量组合的N⁺离子注入黑花生,种植传代后提取OPC,采用紫外-可见分光光度仪和荧光分光光度计分别对不同OPC与不同浓度H₂O₂结合后的光谱进行测量分析.由Lineweaver-Burk方程计算出结合位点数n在0.1—1.56之间;静态猝灭常数KA在10.59—31.94之间. N⁺离子注入黑花生对OPC的结合位点数和猝灭常数有显著影响,为离子注入选育黑花生良种提供有益参考.更多还原

【Abstract】 In order to study the effect of N⁺ ion implantation into black peanuts on the ability of OPC to scavenge oxygen free radicals, N⁺ ions with different energy dose combinations were implanted into black peanuts, and OPC was extracted after planting and passage, using ultraviolet-visible spectrophotometer and fluorescence spectrophotometer respectively The spectra of different OPCs combined with different concentrations of H₂O₂ were measured and analyzed. According to the Lineweaver-Burk equation, the number of binding sites n is between 0.1 and 1.56; the static quenching constant KA is between 10.59 and 31.94. N⁺ ion implantation of black peanut has a significant effect on the number of OPC binding sites and quenching constant, which provides a useful reference for ion implantation to breed black peanut varieties.更多还原