【摘要】 为了解内蒙古地区捻转血矛线虫阿苯达唑耐药虫株Ⅰ型β微管蛋白基因单核苷酸多态性的情况,对来自内蒙古乌审旗、察右后旗和科右前旗3个地区各19条捻转血矛线虫雄虫进行ITS-2基因特异性PCR鉴定为捻转血矛线虫后,扩增出Ⅰ型β微管蛋白基因,测序后对耐药相关167、198和200位点突变情况进行调查分析。结果表明,在这3个地区均未发现167位点突变,主要是198和200位点发生突变,总体上看198位点突变频率高于200位点。分析这57条雄虫基因序列发现4种基因型,分别是198纯合耐药型及200纯合敏感型、198纯合敏感型及200纯合耐药型、198纯合敏感型及200杂合耐药型和198及200均杂合耐药型。而耐药基因型频率乌审旗为100%,察右后旗68.42%,科右前旗94.74%,这与粪便虫卵减少试验结果一致。研究结果为捻转血矛线虫病的预防和治疗提供一个新思路,可有效避免耐药性或者减缓耐药性的产生,减少畜牧养殖行业的经济损失。更多还原

【Abstract】 To understand the situation of single nucleotide polymorphisms(SNP) of isotype-Ⅰ β-tubulin gene of Haemonchus contortus albendazole-resistant strains in Inner Mongolia, the ITS-2 gene of 19 male species of Haemonchus contortus from Uxin Qi, Qahar Youyi Houqi and Horqin Youyi Qianqi in Inner Mongolia was identified by specific PCR as Haemonchus contortus,and isotype-Ⅰ β-tubulin gene was amplified, sequenced and analyzed for investigation of benzimidazole-related SNPs at loci 167,198 and 200.The results showed that F167 Y was not found in these three regions, and E198 A and F200 Y were mainly found.Generally speaking, the frequency of E198 A was higher than that of F200 Y.Analysis of these 57 male gene sequences revealed four genotypes, which were rr-198 and SS-200,SS-198 and rr-200,SS-198 and rS-200,and rS-198 and rS-200.The genotype frequency of drug resistance was 100% in Uxin Qi, 68.42% in Qahar Youyi Houqi and 94.74% in Horqin Youyi Qianqi, which was consistent with FECRT.The results provided a new idea for the prevention and treatment of Haemonchus contortus,which can effectively avoid or slow down the development of drug resistance, and reduce economic losses in the livestock breeding industry.更多还原