【摘要】 本研究探讨了2, 4-表油菜素内酯（2, 4-epibrassinolide, EBR）对盐胁迫下黑麦草（Lolium perenne）种子萌发及幼苗生长相关生理方面的影响。结果表明,在种子萌发过程中,盐胁迫下外源0.01μmol·L^-1的EBR处理,提高了黑麦草种子的发芽率、发芽势、活力指数、株高、鲜重和根系活力,并增强了萌发过程中黑麦草种子的α-淀粉酶活性。在幼苗生长期,盐胁迫下外源0.1μmol·L^-1的EBR处理,使得黑麦草幼苗的超氧化物歧化酶（superoxide dismutase,SOD）、过氧化物酶（peroxidase, POD）、过氧化氢酶（catalase, CAT）和谷胱甘肽过氧化物酶（glutathione peroxidase,GPX）活性显著提高（P <0.05）,而丙二醛（malondialdehyde, MDA）含量显著降低,可溶性糖、可溶性蛋白和叶绿素含量显著升高（P <0.05）。综上所述,外源0.01μmol·L^-1的EBR处理可以缓解100和150 mmol·L^-1 NaCl胁迫对黑麦草种子萌发的抑制效果,外源0.1μmol·L^-1的EBR处理可以缓解250 mmol·L^-1 NaCl胁迫对黑麦草幼苗生长的伤害程度。本研究结果为盐碱地黑麦草的生产和种植提供了一定的理论依据。更多还原

【Abstract】 The physiological effect of 2, 4-epinebrinolide（EBR） on seed germination and seedling growth in Lolium perenne under salt stress was studied. The results showed that the germination rate, germination potential, activity index, plant height,fresh weight, and root activity of ryegrass seeds were improved by under salt stress treatment with exogenous 0.01 μmol·L^-1 EBR. The α-amylase activity of the ryegrass seeds was enhanced during germination. During the seeding growth stage, the activity of superoxide dismutase（SOD）, peroxidase（POD）, catalase（CAT）, and glutathione peroxidase（GPX） in ryegrass seedlings exogenous 0.1 μmol·L^-1 EBR treatment under salt stress significantly increased（P < 0.05）, while the content of malondialdehyde（MDA） significantly decreased. The soluble sugar, soluble protein, and chlorophyll contents were significantly elevated（P < 0.05）. These results indicated that exogenous 0.01 μmol·L^-1 EBR treatment alleviated the inhibitory effect of 100 mmol·L^-1 NaCl and 150 mmol·L^-1 NaCl stress on seed germination, while exogenous 0.1 μmol·L^-1 EBR alleviated the damage of 250 mmol·L^-1 NaCl stress on seedling growth. The results of this study provide a theoretical basis for the application of ryegrass to saline-alkali land.更多还原