【摘要】 为了研究不同浓度茉莉酸甲酯（methyl jasmonate, MeJA）对紫花地丁（Viola philippica）生长及其生理生化的影响,以紫花地丁植株为试验材料,设置5个MeJA浓度（0、1.25、2.5、5、10 mmol·L-1）,喷施紫花地丁叶片背面,研究不同浓度MeJA对紫花地丁叶片的叶螨数、生长指标、抗氧化酶活性、可溶性糖和叶绿素含量等生理指标的影响。结果表明,与未喷施MeJA （CK）相比,外源MeJA能使紫花地丁叶片叶螨的侵染率降低,植株的各类生长指标增加,叶片中过氧化氢酶（catalase, CAT）、过氧化物酶（peroxidase, POD）、超氧化物歧化酶（superoxide dismutase, SOD）、脂氧合酶（lipoxygenase, LOX）的活性增强,叶绿素和可溶性糖含量增加,丙二醛（malondialdehyde, MDA）含量减少。其中,MeJA浓度为10 mmol·L^-1时,紫花地丁叶片的螨害指数为最低,抗满效果最好,而MeJA浓度为2.5 mmol·L^-1时,各类生长指标达到最大。本研究结果表明,外源喷施MeJA通过影响各种酶活性及相应生理指标,提高了紫花地丁对叶螨的抵抗性,有利于其生长发育。更多还原

【Abstract】 In this study, we investigated the effects of different methyl jasmonate（MeJA） concentrations on the growth,physiology, and biochemistry of Viola philippica. We used V. philippica as the experimental materials and sprayed its leaves on the underside with five different MeJA concentrations(0, 1.25, 2.5, 5, and 10 mmol·L^-1) to quantitatively study their effects on the number of Tetranychus mites, growth index, antioxidase activities, and soluble sugar and chlorophyll content.Compared with the non-sprayed MeJA（CK）, exogenous MeJA could reduce the Tetranychus mite infection rate of the leaves of V. philippica. All growth indexes in the plants increased, as well as the activities of catalase, peroxidase, superoxide dismutase, and lipoxygenase, and the chlorophyll and soluble sugar content, while the malondialdehyde content decreased.When the MeJA concentration was 10 mmol·L-1, the mite damage index was the lowest and the anti-overfull effect was the best, while at the MeJA concentration of 2.5 mmol·L^-1, all growth indexes reached the maximum. These results showed that exogenous MeJA could improve the resistance of V. philippica to the mite by affected the activities of various enzymes and corresponding physiological indexes, which was beneficial to its growth and development.更多还原