【摘要】 本工作制备了具有可调控蛋白/细胞作用且低阻抗的功能化聚3,4-乙烯二氧噻吩(PEDOT)的图案化生物界面,并在空间上引导细胞的粘附行为。功能化PEDOT共聚物由具有抗非特异性粘附的磷酸胆碱功能化的EDOT(EDOT-PC)和可进行生物耦合反应的羧基功能化的EDOT(EDOT-COOH)两种单体共聚而成。本工作研究了不同组分共聚物的电化学阻抗性能及其对蛋白、细胞的抗粘附性能,同时通过精氨酸-甘氨酸-天冬氨酸(RGD)多肽的引入实现了在抗非特异性粘附背景下对细胞的特异性粘附。在此基础上,通过光刻及电化学沉积技术制备了由细胞特异性粘附区与抗细胞粘附区组成的图案化PEDOT生物界面,可有效地在空间上控制细胞粘附行为。该工作为研究细胞在材料表面的其他行为提供了可能性,在组织修复、再生工程中有着潜在的应用价值。更多还原

【Abstract】 This study demonstrates that functionalized poly(3,4-ethylenedioxythiophenes)(PEDOTs) with tunable protein/cell interactions and low impedance are applied to fabricate the patterned bio-platform for spatially guiding cell attachment. These functionalized PEDOTs copolymers are prepared by electropolymerized an antifouling EDOT bearing phosphorylcholine(EDOT-PC) and conjugatable EDOT bearing carboxyl acid(EDOT-COOH) into the copolymers. We investigate electrochemical impedance, anti-protein/cell attachment for different composition of the PEDOT copolymers. The specific cell-adhesion on the copolymer surfaces is achieved through grafting RGD peptide on the PEDOTs. Furthermore, we fabricate cell-attachment and cell-repellent micro-patterned PEDOT platforms with different widths by photolithography and electrochemical deposition techniques, the platforms can spatially guide cell attachment effectively, which make it possible for investigating cell behavior. This PEDOT platforms have the potential for tissue repair/regeneration.更多还原