【摘要】 通过测试余甘子提取物的总抗氧化能力和其对弹性蛋白酶的抑制能力,在生化层面考察其抗衰老的功效;人体内的睾酮在5α-还原酶的作用下还原成二氢睾酮,其含量过高是导致雄激素性脱发的首要原因。开发抑制5α-还原酶的植物提取物以降低二氢睾酮含量是缓解雄激素性脱发的有效方式。真皮毛乳头(DP)不仅是毛发生长的信号中心,还表达丰富的5α-还原酶,是毛囊中睾酮发挥作用的靶位点。体外3D培养人毛囊真皮毛乳头细胞(HFDPC)是检测植物提取物防脱生发功效理想的细胞模型。通过测试余甘子提取物的5α-还原酶抑制率、对3D培养HFDPC细胞活性和离体人毛囊生长的影响,在生化、细胞和体外组织层面验证其防脱生发的功效。结果表明,一定质量浓度的余甘子提取物具有抗氧化和弹性蛋白酶抑制作用,还具有抑制5α-还原酶和β-半乳糖苷酶活性、促进HFDPC细胞增殖、增强HFDPC的毛发诱导能力以及促进离体人毛囊生长的功效。更多还原

【Abstract】 Herbal extracts which are safer and more acceptable compared with chemical compounds and hormone products, may contain agonists in the Nrf-2/HO-1 pathway that mobilize the body to produce effective endogenous antioxidant capacity delaying aging and reducing elastase content to keep skin elastic and firm. Antioxidant capacity and elastase inhibition rate of P. emblica extract were tested to obtain its effects on anti-aging. Under the action of 5α-reductase, testosterone is reduced to DHT, whereas high content of DHT is the primary cause of the AGA. Inhibiting 5α-reductase by plant extracts to reduce DHT content is an effective way to alleviate AGA. DP is the signal center of hair growth and the target of testosterone in the hair follicle which expresses rich 5α-reductase. In vitro 3 D cultivation of HFDPC, an ideal detection model, can not only agglomerate them into balls to resemble growth environment in vivo, but also maintain hair induction ability. The 5α-reductase inhibiting ability, the cell viability of HFDPC after 3 D cultivation and in vitro human hair growth rate were examined to acquire its effects on hair growth promotion. The results suggest that P. emblica extract is supposed to delay senescence through inhibition of oxidization and elastase activity, and to facilitate hair growth through inhibition of 5α-reductase and β-galactosidase, enhancement of HFDPC proliferation and inductivity, and promotion of in vitro human hair follicle growth. We evaluate the anti-aging and hair growth promotion effect of the P. emblica to provide reference for further researches.更多还原