【摘要】 为了探究鹅绒委陵菜(Potentilla anserina L.)-丛枝菌根真菌(Arbuscular mycorrhiza fungi,AMF)共生体应答干旱逆境的机制,了解鹅绒委陵菜抗旱机理。本研究以鹅绒委陵菜与根内根生囊霉(Rhizophagus intraradices)为试验材料;采用控制变量的方法,以栽植在未接种根内根生囊霉的灭菌土壤的鹅绒委陵菜为对照组,以栽植在接种根内根生囊霉的灭菌土壤的鹅绒委陵菜为处理组;并分别测定其丙二醛(Malondialdehyde, MDA)含量、超氧化物歧化酶(Superoxide dismutase, SOD)活性、过氧化物酶(Peroxidase, POD)活性及可溶性蛋白含量。结果表明:干旱胁迫会影响土壤中丛枝菌根真菌的正常生长;与未接AMF的鹅绒委陵菜相比,鹅绒委陵菜-AMF共生体在干旱条件下,MDA含量降低、SOD和POD活性升高、可溶性蛋白含量升高,表明AMF能够有效减轻干旱胁迫对鹅绒委陵菜的伤害。更多还原

【Abstract】 In order to explore the mechanism of drought stress response of Potentilla anserina arbuscular mycorrhiza fungi(AMF) symbiosis, and understand the drought resistance mechanism of Potentilla anserina. Potentilla anserina and rhizophagus intraradices were used as experimental materials in this study; Using the method of controlling variables, Potentilla anserina planted in the sterilized soil inoculated with Rhizopus was used as the control group and Potentilla anserina planted in the sterilized soil inoculated with Rhizopus was used as the treatment group; Malondialdehyde(MDA) content, superoxide dismutase(SOD) activity, peroxidase(POD) activity, and soluble protein content were measured. The results showed that drought stress could affect the normal growth of arbuscular mycorrhizal fungi in soil; Compared with Potentilla anserina without AMF,the content of MDA decreased, the activities of SOD and pod increased and the content of soluble protein increased in Potentilla anserina AMF symbiont under drought conditions, indicating that AMF can effectively reduce the damage of drought stress to Potentilla anserina plants.更多还原