【摘要】 为明确宁夏地区玉米镰孢茎腐病病原菌的种类及优势病原菌,本研究于2020年采集宁夏地区玉米(Zea mays L.)茎腐病样品36份,对所采集样品进行组织学分离、纯化和单孢分离,参照Nelson等分类系统进行形态学鉴定,并结合镰孢菌特异性引物EF-1α进行序列分析。本研究共获得209株镰孢菌,包括禾谷镰孢复合种(Fusarium graminearum species complex)、拟轮枝镰孢(F.verticillioides)、木贼镰孢(F.equiseti)、层出镰孢(F.proliferatum)、胶孢镰孢(F.subglutinans)、半裸镰孢(F.incarnatum)、锐顶镰孢(F.acuminatum.)、黄色镰孢(F.culmorum)和燕麦镰孢(F.avenaceum),其分离频率依次为28.2%,23.9%,20.1%,8.1%,7.2%,6.7%,2.9%,1.9%和1.0%。其中,禾谷镰孢复合种在宁夏5市均有分布,且致病性强,为宁夏地区玉米镰孢茎腐病优势致病菌。更多还原

【Abstract】 In order to clarify the species diversity and dominant species of Fusarium causing maize stalk rot in Ningxia, in 2020,36 samples of maize stalk rot in Ningxia were collected. After tissue isolation, purification, and single-spore isolation, the obtained isolates were morphologically identified following Nelson and other classification systems, and molecularly determined according to their EF-1α sequences. The results showed that a total of 209 strains of Fusarium were obtained, including Fusarium graminearum species complex, F. verticillioides, F. equiseti, F. proliferatum, F. subglutinans, F. incarnatum,F. acuminatum,F. culmorum and F.avenaceum,whose isolation frequencies were 28.2%,23.9%,20.1%,8.1%,7.2%,6.7%,2.9%,1.9% and 1.0% respectively. Among them, F. graminearum species complex was distributed in all the five cities of Ningxia, and its pathogenicity was strong, so it was the dominant pathogen of maize stalk rot in Ningxia.更多还原