【摘要】 目的:通过研究不同干预剂量的石榴籽油(pomegranate seed oil, PSO)对骨性关节炎(osteoarthritis, OA)大鼠膝关节Micro CT结构参数的影响以及软骨细胞中II型胶原蛋白(collagen typeⅡ,CoⅡ)、基质金属蛋白酶-1(matrix metalloproteinase, MMP-1)mRNA表达的作用关系,明确石榴籽油改善软骨退变及保护软骨的作用机制。方法:将实验动物分成5组,假手术组(Control)、模型对照组(简称模型组,Model)、实验1、2、3组(Experiment1、2、3)。每组20只动物,除假手术组外其余4个组均通过改良Hulth法建立OA动物模型,驱赶4周,每天给予假手术组和模型组动物1.59 g/kg生理盐水灌胃,3个实验组动物分别给予0.95 g/kg、1.27 g/kg和1.59 g/kg纯石榴籽油灌胃,继续喂养12周后处死,收集大鼠膝关节内侧髁软骨标本,Micro CT分析关节骨体积分数[BV/TV(%)]、结构模型指数(SMI),RT-PCR法检测CoⅡ、MMP-1 mRNA在软骨细胞中的表达。结果:与假手术组比较,模型组和实验1、2、3组BV/TV(%)均减小(P<0.05);与模型组比较,实验1、2、3组BV/TV(%)增加(P<0.05)。与假手术组比较,模型组和实验1、2、3组SMI均增加(P<0.05);与模型组比较,实验1、2、3组SMI减小(P<0.05)。与假手术组比较,模型组MMP-1 mRNA表达量增加(P<0.01);实验1、2、3组与模型组比较,MMP-1 mRNA表达量减少(P<0.01)。与假手术组比较,模型组CoⅡmRNA表达量降低(P<0.01);与模型组比较,实验1、2、3组CoⅡmRNA相对表达量增加(P<0.01)。结论:石榴籽油能够增加骨体积分数,降低结构模型指数,改善软骨下骨骨质疏松状况;石榴籽油能够下调软骨细胞中MMP-1的表达,抑制CoⅡ降解,恢复软骨弹性,延缓关节软骨退变。更多还原

【Abstract】 Objective:To clarify the mechanism of pomegranate seed oil in improving cartilage degeneration and protecting cartilage by studying the effects of pomegranate seed oil(PSO) at different doses on the structural parameters of Micro CT of knee joint in rats with osteoarthritis(OA) and the relationship between the mRNA expression of type II collagen(Coll) and matrix metalloproteinase(MMP-1) in chondrocytes. Methods:The experimental animals were divided into 5 groups: the control group, the model control group, the experiment groups of 1, 2 and 3, 20 animals in every group. Except for the control group, OA animal models of the rest four groups are established through the method of improved Hulth and were driven daily for 4 weeks. The control group and the model control group were intragastrically given 1.59 g/kg saline, and the experiment groups of 1, 2 and 3 were intragastrically given 0.95 g/kg, 1.27 g/kg and 1.59 g/kg of pure pomegranate seed oil respectively and were killed after being kept feeding for 12 weeks, with specimens of rat knee medial condyle cartilage collected, the joint bone volume fraction(BV/TV(%)) and the structure model index(SMI) analyzed by Micro CT and the expression of COLL-II and MMP-1 mRNA in chondrocytes detected by RT-PCR. Results:Compared with that of the control group, BV/TV(%) in the model control group and the experiment groups of 1, 2 and 3 decreased(P<0.05), while BV/TV(%) in the experiment groups of 1, 2 and 3 increased compared with that of the model control group(P<0.05). Compared with that of the control group, SMI in the model control group and the experiment groups of 1, 2 and 3 increased(P<0.05), while SMI in the experiment groups of 1, 2 and 3 decreased compared with that of the model control group(P<0.05). Compared with that of the control group, the expression level of MMP-1 mRNA in the model control group increased(P<0.01), while the expression level of MMP-1 mRNA in the experiment groups of 1, 2 and 3 decreased compared with that of the model control group(P<0.01). Compared with that of the control group, the expression level of Coll mRNA in the model control group decreased(P<0.01), while the relative expression level of Coll mRNA in the experiment groups of 1, 2 and 3 increased compared with that of the model control group(P<0.01). Conclusion:Pomegranate seed oil can increase bone volume fraction, reduce structural model index, and improve subchondral osteoporosis. Pomegranate seed oil can down-regulate the expression of MMP-1 in chondrocytes, inhibit the degradation of Coll, restore cartilage elasticity, and delay the degeneration of articular cartilage.更多还原