【摘要】 【目的】建立方便可行的伪狂犬病毒血清抗体的酶联免疫吸附测定方法,并可特异性鉴别伪狂犬病毒TK~-/gG~-基因缺失疫苗免疫株和野毒株。【方法】利用原核表达系统表达伪狂犬病毒gG抗原片段,蛋白纯化后包被,优化反应条件,建立一种用于检测伪狂犬病毒gG抗体的间接酶联免疫吸附测定方法。【结果】通过不同血清样品的检测试验,该酶联免疫吸附测定方法可以鉴别伪狂犬病毒TK~-/gG~-基因缺失疫苗免疫猪和感染野毒的血清学阳性猪。【结论】成功建立了检测伪狂犬病毒gG抗体的酶联免疫吸附测定方法,并可特异鉴别伪狂犬病毒TK~-/gG~-基因缺失疫苗免疫株和野毒株,此方法敏感性强,特异性高,操作简便,适合于临床大量检测。更多还原

【Abstract】 【Objective】 The purpose of this study is to establish a convenient enzyme linked immunosorbent assay method for detection the serum antibody of pseudorabies virus, and to identify the Pseudorabies Virus TK~-/gG~- gene deletion vaccine strain and the wild strain specifically. 【Methods】 In this study, pseudorabies virus partial gG antigen was expressed in prokaryotic expression system. Using the purified gG protein as the coating antigen. an indirect enzyme linked immunosorbent assay method was developed to detect pseudorabies virus gG antibody after optimized the reaction conditions. 【Results】 By testing different serum samples, it was proved that this enzyme linked immunosorbent assay method could identify pseudorabies virus TK~-/gG~- gene deletion vaccine immunized swine and wild-type virus infected swine. 【Conclusion】 We established an enzyme linked immunosorbent assay method for pseudorabies virus gG antibody detection, which can identify the pseudorabies virus TK~-/gG~- gene deletion vaccine strain and the wild strain specifically. This enzyme linked immunosorbent assay is sensitive, specific and easy to operate, and suitable detecting to clinical samples.更多还原