【摘要】 以西兰花为试材，采用气调处理的方法，以自然空气为对照（CK）,设置20%O₂+20%CO₂、50%O₂+20%CO₂、80%O₂+20%CO₂的气体比例，研究了20℃贮藏条件下O₂对西兰花的贮藏品质和叶绿素降解的影响，阐明O₂促进西兰花黄化的作用机制，以期为研究高O₂条件下叶绿素的降解调控提供参考依据。结果表明：高O₂比例降低了西兰花体内叶绿素、维生素C以及可溶性蛋白质含量；气调处理中O₂比例提高能够促进叶绿素b还原酶编码基因（BoNYC1）、滞绿蛋白编码基因（BoSGR）、脱镁叶绿素酶编码基因（BoPPH）、脱镁叶绿酸a氧化酶编码基因（BoPaO）、红色叶绿素降解产物还原酶编码基因（BoRCCR）表达水平，尤其是BoSGR、BoPPH和BoRCCR相对表达量高峰提前出现，促进了叶绿素分解代谢，加速了西兰花黄化进程。其中，O₂促进叶绿素降解过程中BoPPH基因表达可能是促进黄化的主要原因，SGR则通过互作影响BoPPH表达。更多还原

【Abstract】 Taking broccoli as test materials, the air conditions of 20%O₂+20%CO₂,50%O₂+20%CO₂ and 80%O₂+20%CO₂ were employed as treatments, natural air was set as control（CK）.During storage at 20 ℃,the storage quality of broccoli florets and the relative expression of genes related to chlorophyll degradation were determined.To explore the effects of O₂ on the degradation of broccoli chlorophyll in controlled atmosphere.The results of this experiment could provide a theoretical basis for the regulation of chlorophyll degradation under high O₂ conditions.The results showed that with the increase of O₂ ratio, the chlorophyll b reductase encoding gene（BoNYC1）,the chlorophyll encoding gene（BoSGR）,the pheophytinase encoding gene（BoPPH）,the pheophorbide a oxidase encoding gene（BoPaO）,red chlorophyll degradation product reductase encoding gene（BoRCCR） expression levels and their relative expression peaks appeared earlier, which promoted chlorophyll catabolism and accelerated the yellowing process of broccoli.Among them, O₂ promoted the gene expression of BoPPH in the process of chlorophyll degradation, which may be the main reason for promoting yellowing, SGR interacted the expression of BoPPH.更多还原