【摘要】 目的:探讨丙型肝炎病毒核心抗原(hepatitis C virus core antigen, HCV-cAg)与丙型肝炎病毒RNA(hepatitis C virus RNA,HCV-RNA)关系以及丙氨酸氨基转移酶(ALT)、天门冬氨酸氨基转移酶(AST)、谷氨酰转肽酶(GGT)水平与HCV-RNA病毒载量之间的相关性。方法:选取78例HCV抗体检测阳性的丙型肝炎或疑似丙型肝炎病人作为观察组,另选取同期健康体检者20名作为对照组。采用ELISA法和荧光定量PCR法等对2组血清样本进行HCV-cAg、HCV-RNA和ALT、AST、GGT水平测定,并比较各参数之间的关系。结果:观察组HCV-cAg阳性检出率为41.0%(32/78),HCV-RNA阳性检出率为53.8%(42/78),差异有统计学意义(P<0.01),且二者之间具有较好一致性(κ=0.747)。观察组ALT、AST和GGT水平均明显高于对照组(P<0.01),但与HCV-RNA病毒载量均无明显相关性(P>0.05)。结论:HCV-cAg和HCV-RNA在检测丙型肝炎方面有较好一致性和相关性,可为丙型肝炎的临床诊断提供有价值的依据。更多还原

【Abstract】 Objective:To investigate the relationship between hepatitis C virus core antigen(HCV-cAg) and hepatitis C virus RNA(HCV-RNA),and association between levels of alanine aminotransferase(ALT),aspartate aminotransferase(AST) and glutamyl transpeptidase(GGT),and HCV-RNA viral load.Methods:Seventy-eight patients with positive HCV antibody or suspected hepatitis C were divided into the observation group, and another 20 healthy examinees were set as the control group during the same period.The serum levels of HCV-cAg, HCV-RNA,ALT,AST and GGT in two groups were determined by ELISA and fluorescence quantitative PCR,and the relationship among the above parameters were compared.Results:In the observation group, the positive detection rate of HCV-cAg was 41.0%(32/78),the positive detection rate of HCV-RNA was 53.8%(42/78),the difference of which was statistically significant(P<0.01),and there was a good consistency between the two groups(κ=0.747).The levels of ALT,AST and GGT levels in observation group were significantly higher than those in control group(P<0.01),but there was no significant correlation with HCV RNA viral load(P>0.05).Conclusions:The HCV-cAg and HCV-RNA have good consistency and correlation in the detection of hepatitis C,and can provide valuable basis for the clinical diagnosis of hepatitis C.更多还原