【摘要】 目的:研究由金银花、黄连、甘草等组成的复方中草药制剂对青脚麻鸡胸腺显微结构、免疫功能以及凋亡相关基因Caspase-3 mRNA和蛋白表达的影响。方法:选用125羽健康28 d未接种法氏囊疫苗的青脚麻鸡,按照体重相近原则随机分对照组、攻毒组和试验Ⅰ～Ⅲ组,每组5个重复(每个重复5羽),对照组和攻毒组饲喂基础饲粮,试验Ⅰ～Ⅲ组分别添加0.5%、1.0%和1.5%的复方中草药制剂。攻毒组和试验组在30 d通过点眼人工感染IBDV,试验期为7 d。结果:与对照组相比,IBDV攻毒第3天,青脚麻鸡胸腺指数、Caspase-3 mRNA和蛋白相对表达量以及胸腺单位面积Caspase-3阳性细胞数量均显著升高(P<0.05),而胸腺皮质厚度和细胞因子(IL-2、INF-γ)水平均显著降低(P<0.05);相比攻毒组,试验Ⅱ组青脚麻鸡胸腺指数、Caspase-3 mRNA和蛋白相对表达量均显著降低(P<0.05),而皮质厚度和IL-2含量均显著升高(P<0.05)。IBDV攻毒第7天,与对照组相比,青脚麻鸡胸腺指数、Caspase-3 mRNA表达量和胸腺单位面积Caspase-3阳性细胞数显著升高(P<0.05),而胸腺皮质厚度和细胞因子(IL-2、INF-γ)水平均显著降低(P<0.05);相比攻毒组,试验Ⅱ组青脚麻鸡胸腺指数、Caspase-3 mRNA相对表达量和胸腺单位面积Caspase-3阳性细胞数均显著降低(P<0.05)。显微观测发现,IBDV攻毒第3天和第7天,与对照组相比,试验Ⅱ组青脚麻鸡胸腺显微结构无明显变化,皮质和髓质结构清晰,细胞排列紧密。结论:饲粮添加1.0%复方中草药制剂对IBDV感染青脚麻鸡胸腺显微结构有明显改善作用,并能提高胸腺免疫,抑制胸腺淋巴细胞凋亡,减轻IBDV对青脚麻鸡胸腺的损伤。更多还原

【Abstract】 Objective: To explore the effects of compound Chinese herbal medicine(such as honeysuckle, coptis, licorice, etc.) on microstructure, immune function of Partridge Shank chicken thymus and the expression of apoptosis-related gene Caspase-3 mRNA and protein. Methods: According to the principle of similar body weight, 125 healthy 28-day-old Partridge Shank chicken that were not vaccinated with IBD vaccine were randomly divided into control group, the challenge group and experiment group Ⅰ-Ⅲ, with 5 replicates in each group(5 per repeat). The control group and the challenge were fed on basal diets, and the experiment groups Ⅰ-Ⅲ were fed on 0.5%, 1.0% and 1.5% compound Chinese herbal medicine respectively. The challenge group and the experiment group were infected with IBDV through eye drops at the age of 30-day-old, and the experiment period was 7 d. Results: Compared with the control group after 3 days of IBDV challenge, the thymus index, relative expression of Caspase-3 mRNA and protein, and the number of Caspase-3 positive cells of Partridge Shank chicken of challenge group were significantly increased(P<0.05), but the thickness of thymus cortex, cytokine(IL-2, INF-γ) were significantly reduced(P<0.05). Compared with the challenge group, the thymus index and the relative expression of Caspase-3 mRNA and protein in experiment group II were significantly reduced(P<0.05), but the cortical thickness and IL-2 content were significantly increased(P<0.05). Compared with the control group after 7 days of challenge, the thymus index, Caspase-3 mRNA and Caspase-3 positive cells of thymus in the challenge group were significantly increased(P<0.05), but the cortical thickness and cytokine(IL-2, INF-γ) content were significantly reduced(P<0.05). Compared with the challenge group, the thymus index, Caspase-3 mRNA and the number of Caspase-3 positive cells of Partridge Shank chicken of the experiment group Ⅱ were significantly decreased(P<0.05). Microscopic observations showed that compared with the control group, the microstructure of Partridge Shank chicken thymus in experiment group II had no obvious changes after the 3rd and 7th day of IBDV challenge, the cortex and medulla structure were clear, and the cells were tightly arranged. Conclusion: Adding 1.0% compound Chinese herbal medicine to the diet can significantly improve the thymus structure of Partridge Shank chicken infected with IBDV, improve the immune function of the thymus, inhibit the apoptosis of thymic lymphocytes, and reduce the effects of IBDV on the thymus.更多还原