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黑皮鸡枞的分子鉴定与营养需求研究

Study of Molecular Identification and Nutritional Requirement of Hymenopellis raphanipes

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【作者】 罗影; 关永强; 贾培松; 赵振豪; 努尔孜亚·亚力买买提; 魏鹏; 贾文捷;

【Author】 LUO Ying;GUAN Yong-qiang;JIA Pei-song;Institute of Plant Protection, Xinjiang Academy of Agricultural Sciences;Xinjiang Institute of Chinese Materia Medica and Ethnodrug;

【通讯作者】 贾文捷;

【机构】 新疆农业科学院植物保护研究所/农业部西北荒漠绿洲作物有害生物综合治理重点实验室; 新疆维吾尔自治区中药民族药研究所; 新疆农业大学;

【摘要】 为了明确黑皮鸡枞的营养需求,促进其制种工艺的提高。以新疆麦盖提县人工栽培的菌株为试材,采用单因素试验分别对其母种和原种的营养需求进行试验。结果显示,黑皮鸡枞母种培养基中最佳碳源为麦芽糖,最佳氮源为酵母浸粉,加入1%的MgSO4、CaCl2及枣粉均能促进其菌丝生长,而NaCl和KH2PO4抑制其菌丝生长,菌丝培养最适温度为20~28℃,最适pH为5.5~6.0;原种培养基中将棉籽壳与木屑以7∶3比例混合作为主料使用效果明显。

【Abstract】 To identify the effects of different nutrients on the mycelical growth of Hymenopellis raphanipes, and improve the level of strains production.The test was studied by single factor plate experiment, using a Hymenopellis raphanipes strain from Maigaiti County of Xinjiang as material.The results showed that the best carbon and nitrogen source were maltose and yeast extract respectively.The addition of 1% MgSO4, CaCl2 and jujube powder could promote the mycelial growth, while NaCl and KH2PO4 inhibited it.The optimal temperature range was 20-28 ℃, and the optimal pH was 5.5-6.0.The mixture of cottonseed hull and sawdust at the ratio of 7∶3 was used as the main material.

【关键词】 黑皮鸡枞; ITS; 营养需求;
【Key words】 Hymenopellis raphanipes; ITS; Nutritional requirement;
【基金】 国家现代农业产业技术体系项目(CARS-20)
  • 【文献出处】 安徽农业科学 ,Journal of Anhui Agricultural Sciences , 编辑部邮箱 ,2021年16期
  • 【分类号】S646
  • 【被引频次】3
  • 【下载频次】229
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