【摘要】 [目的]研究不同种类和浓度的抗生素对卷叶贝母鳞片叶诱导生长的影响。[方法]分别将不同浓度的头孢霉素、头孢曲松钠和头孢噻肟钠抗生素添加到卷叶贝母鳞片叶的生长培养基中进行诱导培养,研究不同种类和浓度的抗生素对卷叶贝母鳞片叶生长的影响。[结果]不同浓度的3种抗生素对卷叶贝母鳞片叶诱导生长作用的影响是不同的。与对照试验比较,在培养基中添加不同浓度的头孢霉素对其愈伤组织的诱导均有抑制作用,以添加浓度为800 mg/L的头孢霉素对其愈伤组织的抑制作用最强,其愈伤组织的诱导率最低,仅为25.18%,但当其添加浓度为200 mg/L时,芽的诱导率最高,为47.50%;在培养基中添加头孢曲松钠的浓度为200 mg/L时,其卷叶贝母鳞片叶的愈伤组织诱导率和芽诱导率均最高,分别为100%和82.52%;在培养基中添加400 mg/L的头孢噻肟钠时,诱导愈伤组织的效果最好,诱导率为100%;而在添加浓度为200 mg/L时,其芽诱导率最高,为92.53%。[结论]该研究为下一步开展卷叶贝母植物遗传转化工作提供理论依据。更多还原

【Abstract】 [Objective]To study the effects of antibiotics under different kinds and concentrations on the induced growth of Fritillaria cirrhosa scaly leaf.[Method]Different concentrations of cephalosporin, ceftriaxone sodium and cefotaxime sodium antibiotics were added to the growth medium of Fritillaria cirrhosa scaly leaves for induction culture, to study the effects of different kinds and concentrations of antibiotics on the growth of Fritillaria cirrhosa’s scales.[Result]Different concentrations of 3 antibiotics had different effects on the growth induction of Fritillaria cirrhosa scaly leaf. Compared with the control experiment, adding different concentration of cephalosporins in culture toxin on the callus induction has inhibitory effect, to add a concentration of 800 mg/L of cephalosporins drug the strongest inhibitory effect of the callus, the callus induction rate lowest was only 25.18%, but when added concentration of 200 mg/L, bud induction rate was as high as 47.50%;when ceftriaxone sodium was added to the medium at a concentration of 200 mg/L, the callus induction rate and bud induction rate of Fritillaria cirrhosa scales were at the highest of 100% and 82.52% respectively; when 400 mg/L cefotaxime sodium was added to the medium, the callus induction effect was the best, with the induction rate of 100%;when the concentration was 200 mg/L, the highest bud induction rate was 92.53%.[Conclusion]This research provides a theoretical basis for the next step of genetic transformation of Fritillaria cirrhosa.更多还原