【摘要】 [目的]评估苦参碱对变形链球菌致龋性(如生长、产酸、生物被膜形成和细菌代谢活性)的影响。[方法]通过结晶紫染色测定苦参碱对生物被膜形成的抑制作用与已成熟生物被膜的根除作用,通过pH计测定苦参碱对变形链球菌产酸的影响,通过MTT法测定了苦参碱对变形链球菌浮游细胞与生物被膜细胞代谢活性的影响。[结果]苦参碱能够显著抑制变形链球菌的生长与生物被膜的形成,对已成熟的生物被膜也有根除作用。苦参碱处理后变形链球菌有机酸的产生明显减少,同时变形链球菌浮游细胞和生物被膜细胞的代谢活性也受到了抑制。[结论]苦参碱可以抑制致龋性生物被膜,是预防龋齿的候选药物之一。更多还原

【Abstract】 [Objective]To evaluate the effect of matrine on the cariogenicity of Streptococcus mutans(such as growth, acid production, biofilm formation and bacterial metabolic activity).[Method]The inhibitory effect of matrine on the formation of biofilms and the eradication of mature biofilms were measured by crystal violet staining.The effect of matrine on the acid production of S.mutans was measured by a pH meter.The effect of matrine on the metabolic activities of S.mutans planktonic cells and biofilm cells was determined by MTT method.[Result]Matrine could significantly inhibit the growth of S.mutans and the formation of biofilms, and it could also eradicate mature biofilms.After treatment with matrine, the production of organic acids by S.mutans was significantly reduced, and the metabolic activities of it’s planktonic cells and biofilm cells were also inhibited.[Conclusion]Matrine can inhibit cariogenic biofilms and is one of the candidate drugs for preventing dental caries.更多还原