【摘要】 通过分离培养张家口某鸡场MS菌株,采用PCR方法扩增MS 16S RNA,并对分离培养菌株进行药敏试验。结果显示,感染MS肉鸡站立困难,跗关节和爪垫肿胀,分离培养的病原体菌落为光滑、圆形,小面积"煎蛋样"。PCR产物在207 bp处出现条带。药敏试验发现该菌落对酒石酸泰乐菌素和延胡索酸泰妙菌素敏感,最小抑菌浓度分别为0.122和0.244μg/mL。因此,可确定该鸡场已感染滑液囊支原体,推荐使用酒石酸泰乐菌素或延胡索酸泰妙菌素作为首选抑菌治疗药物。更多还原

【Abstract】 Mycoplasma synoviae(MS)strains were isolated from a chicken farm in Zhangjiakou and cultured, PCR method was used to amplify 16 S RNA of MS. And drug sensitivity test was made on the isolated stains. The results showed that broilers infected with MS had difficulty in standing, swelling of the tarsal joints and paw pads, and the isolated and cultured pathogen colonies were smooth, round, with "fried egg-like" small areas. PCR product showed a band at 207 bp. The drug susceptibility test showed that the colony was sensitive to tylosin tartrate and tiamulin fumarate, and the minimum inhibitory concentrations were 0.122 and 0.244 μg/mL, respectively. Therefore, it could be determined that the chicken farm was infected with MS, and it was recommended to use tylosin tartrate or tiamulin fumarate as the first antibacterial medicine.更多还原