【摘要】 以小美旱杨一年生幼嫩茎段为外植体,研究了不同消毒时间、不同基本培养基类型、不同生长调节剂配比下小美旱杨初代培养的情况。结果表明:茎段表面消毒以75%乙醇30 s+0.1%HgCl₂ 12 min为佳;MS培养基中腋芽的萌发率高于1/2MS和WPM培养基;初代培养最适的生长调节剂为0.10 mg/L 6-BA+0.10 mg/L NAA,诱导率为91.67%。更多还原

【Abstract】 Taking the stems as explants, the disinfection methods, basic medium and different hormone affecting the initial culture of Populus popular’s were studied. The results showed that the best disinfection method was using 0.1%HgCl₂ for 12 minutes, it had lower contaminated rate and mortality rate. MS medium was the best one for primary culture, the germination time and growth conditions were better than other mediums. The highest percentage of auxillary bud induction（91.67%） was obtained on MS medium with 0.10 mg/L 6-BA and 0.10 mg/L NAA.更多还原