【摘要】 基于T-Hg²⁺-T结构,在纳米金修饰电极（AuNPs/Au）表面构建了一种灵敏检测Hg²⁺的电化学生物传感器.首先,在金（Au）表面电沉积AuNPs作为基底,增加ssDNA的引入量,50℃下硫堇（Th）依靠T和Hg²⁺的特异性结合迅速嵌入ssDNA双螺旋内部,基于插入的电化学信号测定Hg²⁺.在最佳条件下,传感器对Hg²⁺检测的线性范围为10～100 nmol·L^-1,检出限低至7.4 nmol·L^-1.传感器展现出良好的选择性.更多还原

【Abstract】 A sensitive electrochemical biosensor for detection of Hg²⁺ was prepared on the surface of AuNPs/Au based on the T-Hg²⁺-T structure. Firstly, AuNPs were electrodeposited on the surface of Au, which increased the amount of ssDNA introduced. At 50 ℃, thionine（Th） was quickly embedded in the ssDNA double helices by the specific binding of T and Hg²⁺. Hg²⁺ was determined by the insertion of electrochemical signals. Under the optimal conditions, the linear range of the biosensor to Hg²⁺ was in the range of 10-100 nmol·L^-1, and the detection limit was as low as 7.4 nmol·L^-1. The sensor showed good selectivity.更多还原