【摘要】 目的应用不同化疗药物建立卵巢早衰动物模型,探索有效的卵巢早衰动物模型建立方法。方法采用两种临床常用化疗药物紫杉醇、环磷酰胺诱导处理动情周期正常的雌性Wister大鼠,分为紫杉醇组与紫杉醇对照组、环磷酰胺组与环磷酰胺对照组。一段时间后观察大鼠一般生理情况,检测各组大鼠体重的变化,应用ELISA技术检测各组血清中E2、FSH的表达情况,同时比较各组卵巢组织形态、卵泡数的变化。结果环磷酰胺组较对照组,大鼠活动减少,体重下降,E2水平明显下降(P<0.05),FSH水平明显升高(P<0.05),卵巢结构萎缩,卵泡数明显减少。紫杉醇组与对照组大鼠相比,雌激素含量明显降低,活动量未见明显减少,体重均呈增加趋势,卵巢结构、卵巢长径、卵泡数以及促卵泡素均未见明显差别。结论环磷酰胺在建立卵巢早衰的动物模型中比紫杉醇具有优势,更适用于卵巢早衰相关研究。更多还原

【Abstract】 Objective To establish animal models of premature ovarian failure using different chemotherapy drugs, and to explore the establishment of stable animal models of premature ovarian failure. Methods Two common chemotherapeutic drugs, paclitaxel and cyclophosphamide, were used to induce female wister rats with normal estrous cycles. They were divided into paclitaxel group, paclitaxel control group, cyclophosphamide group and cyclophosphamide control group. After a period of time, the general physiology of the rats were observed, and the changes in the body weight of each group were detected. ELISA technique was used to detect the expression of E2 and FSH in the serum of each group, and the changes in the morphology of the ovaries and the number of follicles were compared. Results Compared with the control group, the cyclophosphamide group had reduced activity, weight loss, lower E2 level(P < 0.05), higher FSH level(P < 0.05), atrophy of the ovaries, and significant reduction in the number of follicles. Compared with the control group, the paclitaxel group showed significant decrease in estrogen content and increase in body weight. There were no significant differences in activity, ovarian structure, ovarian length, follicle number, and follicle-stimulating hormone. Conclusion Cyclophosphamide has advantage over paclitaxel in establishing an animal model of premature ovarian failure and is more suitable for studies related to premature ovarian failure.更多还原